Tetrasodium 4-amino-5-hydroxy-6-[[2-methoxy-5-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]-3-[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 31, 2015 - April 22, 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

no

GLP compliance:

no

Other quality assurance:

ISO/IEC 17025 (General requirements for the competence of testing and calibration laboratories)

Type of method:

air comparison pycnometer (for solids)

Key result

Type:

relative density

Density:

1.59

Temp.:

20.1 °C

Results

According to the certificate of analysis, the test item contained approx. 7.2 % water. Therefore, the test item was dried under vacuum at 50 °C for about 42 h until the weight of the test item was constant.

A measurement chamber with a volume of V_c = 7.8961 cm³ and an expansion chamber with a volume of V_r = 6.0848 cm³ were used. Two test series with different weights were performed at ambient temperature (20.1 °C).The measurements showed a slow pressure decrease within the closed measurement chamber after the application of Helium. This pressure decrease was assumed to result from a gas adsorption of the test item. Because of the observed pressure decrease, the measurements were performed after an adjustment time to reach a constant pressure (about 13 to 30 minutes before measuring p₁and about 7 to 12 minutes before measuring p₂). For results see Table 1and Table 2.

Table 1: Results of the 1^st test series

Weight: 1.1573 g		p1/ psi		p2/ psi		Volume / cm^3		Density rPR/ g·cm^-3
1^stmeasurement		19.670		10.638		0.7293		1.5869
2^ndmeasurement		19.707		10.660		0.7264		1.5933
3^rdmeasurement		19.661		10.635		0.7265		1.5929
Mean						0.7274		1.5910

Table 2: Results of the 2^nd test series

Weight: 1.2067 g		p1/ psi		p2/ psi		Volume / cm^3		Density rPR/ g·cm^-3
1^stmeasurement		19.506		10.530		0.7578		1.5924
2^ndmeasurement		19.583		10.574		0.7542		1.6000
3^rdmeasurement		19.636		10.601		0.7566		1.5950
Mean						0.7562		1.5958

Mean density r_PR for the two test series: 1.5934 g/cm³

Relative density (compared to water at 4 °C) D₄^R : 1.59  (20.1 °C)

 

 

Executive summary:

The relative density at ambient temperature (compared to water at 4 °C) was determined to be:

D₄^R = 1.59      (20.1 °C)

Description of key information

The density of the test item was determined according to the European Commission Regulation (EC) No. 440/2008, Method, A.3. Density of liquids and solids: Gas comparison pycnometer and according to the OECD test guideline, OECD 109 Density of liquids and solids.

Key value for chemical safety assessment

Relative density at 20C:

1.59