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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable well-documented study report which meets basic scientific principles.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Cross-reference
Reason / purpose:
read-across: supporting information
Reference
Endpoint:
basic toxicokinetics in vivo
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Study period:
1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable well-documented study report which meets basic scientific principles.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across source
Principles of method if other than guideline:
Labeled paraffins with 8-18 C atoms prepared from unsaturated hydrocarbons by addition of deuterium have been added in oily solution to normal rats’ food.
GLP compliance:
not specified
Radiolabelling:
yes
Species:
rat
Route of administration:
oral: feed
Details on absorption:
No information
Details on distribution in tissues:
Deuterium was found in the fatty acids of the body fats and the liver lipids especially after feeding octadecane and hexadecane
Details on excretion:
No information
Details on metabolites:
oleic, stearic, and palmitic acids containing deuterium have been isolated
Conclusions:
Interpretation of results: no data
After six days an increase of deuterium content in the body fluid of all the rats was observed indicating that the labeled compounds had been metabolized.
Executive summary:

Labeled paraffins with 8-18 C atoms prepared from unsaturated hydrocarbons by addition of deuterium have been added in oily solution to normal rats’ food. After six days an increase of deuterium content in the body fluid of all the rats was observed indicating that the labeled compounds had been metabolized. Deuterium was found in the fatty acids of the body fats and the liver lipids especially after feeding octadecane and hexadecane. Isolating oleic, stearic, and palmitic acids containing deuterium, indicated that methyl- and beta-oxidation of these hydrocarbons has occurred. Fatty acids resulting from the metabolism of hydrocarbons with shorter chains were not deposited but in these cases the urine contained fatty acids with higher deutrium content than after administration of octadecane and hexadecane. According to the deuterium content of the neutral fractions from the liver and body lipids all the hydrocarbons tested were deposited only to a small extent, the largest depots occurring mainly after feeding with octadecane and hexadecane.

Data source

Reference
Reference Type:
publication
Title:
Demonstration of oxidation of a naphthenic hydrocarbon Dodecylcyclohexane in rats
Author:
Tulliez J., Peleran J. C.
Year:
1977
Bibliographic source:
Febs Letters 75(1): 120-122

Materials and methods

Principles of method if other than guideline:
Labeled paraffins with 8-18 C atoms prepared from unsaturated hydrocarbons by addition of deuterium have been added in oily solution to normal rats’ food.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Paraffins with 8-18 C atoms prepared from unsaturated hydrocarbons
Radiolabelling:
yes

Test animals

Species:
rat

Administration / exposure

Route of administration:
oral: feed

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on absorption:
No information
Details on distribution in tissues:
Deuterium was found in the fatty acids of the body fats and the liver lipids especially after feeding octadecane and hexadecane
Details on excretion:
No information

Metabolite characterisation studies

Details on metabolites:
oleic, stearic, and palmitic acids containing deuterium have been isolated

Applicant's summary and conclusion

Conclusions:
Interpretation of results: no data
After six days an increase of deuterium content in the body fluid of all the rats was observed indicating that the labeled compounds had been metabolized.
Executive summary:

Labeled paraffins with 8-18 C atoms prepared from unsaturated hydrocarbons by addition of deuterium have been added in oily solution to normal rats’ food. After six days an increase of deuterium content in the body fluid of all the rats was observed indicating that the labeled compounds had been metabolized. Deuterium was found in the fatty acids of the body fats and the liver lipids especially after feeding octadecane and hexadecane. Isolating oleic, stearic, and palmitic acids containing deuterium, indicated that methyl- and beta-oxidation of these hydrocarbons has occurred. Fatty acids resulting from the metabolism of hydrocarbons with shorter chains were not deposited but in these cases the urine contained fatty acids with higher deutrium content than after administration of octadecane and hexadecane. According to the deuterium content of the neutral fractions from the liver and body lipids all the hydrocarbons tested were deposited only to a small extent, the largest depots occurring mainly after feeding with octadecane and hexadecane.