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EC number: 275-156-8 | CAS number: 71048-82-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant, guideline study, available as unpublished report, no restrictions, fully adequate for assessement.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 1-(2,6,6-trimethyl-3-cyclohexen-1-yl)-2-buten-1-one
- EC Number:
- 260-709-8
- EC Name:
- 1-(2,6,6-trimethyl-3-cyclohexen-1-yl)-2-buten-1-one
- Cas Number:
- 57378-68-4
- IUPAC Name:
- 1-(2,6,6-trimethylcyclohex-3-en-1-yl)but-2-en-1-one
- Details on test material:
- Purity: 98.9%
Lot number: SM/4051089
Expiration date: 9/2006
Physical Description: Clear colorless liquid
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Jackson Laboratories, Bar Harbor, ME 04609
- Age at study initiation: 11 weeks at start of dosing; records of dates of birth for animals used in this study are retained in the Calvert archives.
- Weight at study initiation: 18-25 grams at the outset (Day 1) of the study
- Housing: Animals were group housed (5 per cage) upon receipt in compliance with National Research Council "Guide for the Care and Use of Laboratory Animals". The room in which the animals were kept was documented in the study records. No other species were kept in the same room.
- Diet (e.g. ad libitum): Animals had access to Certified Rodent Chow 7012C ad libitum. The lot number(s) andspecifications of each lot used are archived at Calvert. No contaminants were known to be present in the certified diet at levels that would be expected to interfere with the results of this study. Analysis of the diet was limited to that performed by the manufacturer, records of which are maintained in the Calvert archives.
- Water (e.g. ad libitum): Tap water was available ad libitum, via water bottles. The water is routinely analyzed for contaminants as per Calvert SOP's. No contaminants were known to be present in the water at levels that would be expected to interfere with the results of this study. Results of the water analysis are maintained in the Calvert archives.
- Acclimation period: Study animals were acclimated to their housing for six days prior to their first day of dosing
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21.1 to 26.7 °C
- Humidity (%): 30-53
- Air changes (per hr): no information
- Photoperiod (hrs dark / hrs light): 12 hours light/12 hours dark
Study design: in vivo (LLNA)
- Vehicle:
- other: Diethyl phthalate/ethanol in a 3:1 ratio
- Concentration:
- 7.5%, 15%, 30%
- No. of animals per dose:
- 5 animals
- Details on study design:
- Mice were treated on the dorsal surface of both ears, once per day on Days 1, 2, and 3. Approximately 24±2 hours between applications of test article was maintained. On Day 6 the mice were injected i.v, with 20 μCi of 3H_ thymidine in 250 μI of sterile saline. Five hours later the mice were euthanized by CO2 asphyxiation. Ear thickness measurements of each mouse were recorded and the draining auricular lymph nodes removed. At removal, the number of nodes collected per animal was recorded, and the nodes were examined for size/appearance and the data recorded. Any unexpected observations were noted in study records. A single cell suspension was prepared from the lymph nodes of each mouse. Cells were washed twice with phosphate buffered saline (PBS) and precipitated with 5% trichloroacetic acid (TCA) overnight at 2-8°C. The pellets were recovered by centrifugation and resuspended in 1 ml of TCA and transferred to a vial containing scintillation fluid. An additional 1 ml of TCA was used to rinse the tube, and it was also transferred to the scintillation fluid. Incorporation of 3H_ thymidine was measured in a ß-scintillation counter.
- Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- The mean disintegrations per minute (DPM) for each group was evaluated using SYSTAT version 9.01, developed by SPSS, Inc. Increases in 3H-thymidine incorporation relative to the vehicle-treated control were derived for each group and recorded as stimulation indices (SI). The criterion for a positive response is that one or more concentrations of a test article elicits a 3-fold or greater increase in isotope incorporation relative to the vehicle control.
Body weight data and ear thickness measurements were also evaluated.
Individual DPM values were analyzed by log transformation (base 10) of the data. The evaluation of the equality of means for the DPM, body weight and ear thickness data was made by a one-way analysis of variance using the F distribution to assess statistical significance. If statistically significant differences between the means are found, a Dunnett's test was used to determine the degree of significance from the control means.
Results and discussion
- Positive control results:
- The positive control, 35% (v/v) HCA, resulted in a stimulation index (SI) of 6.66. A 3-fold or greater increase in proliferative activity relative to the concurrent vehicle treated control is considered a positive response. In addition, the response with the positive control in this study was also statistically significant (p<0.01) when compared to the vehicle control group.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: see Remark
- Remarks:
- Exposure to the test substance at 7.5, 15 and 30% (w/v) resulted in stimulation indices of 1.95, 5.70, and 4.24, respectively. Statistically significant differences were also found at 15 and 30% (P < 0.01 and P < 0.05, respectively).The EC3 was calculated to be 9.6%.
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: The mean DPM for each treatment group was compared to the vehicle control group. Since the data indicated that the test article was positive, the EC3 was calculated to be 9.6.
Any other information on results incl. tables
Group |
Treatment |
Dose |
DPM (mean ± sem) |
S1 (Test/control Ratio) |
Results** |
1 |
Vehicle* |
- |
228 ± 28 |
- |
- |
2 |
04-230(6) |
7.5% |
444 ± 228 |
1.95 |
- |
3 |
04-230(6) |
15% |
1299 ± 336**** |
5.70 |
+ |
4 |
04-230(6) |
30% |
966 ± 235*** |
4.24 |
+ |
5 |
HCA |
35% |
1518±221**** |
6.66 |
+ |
* Diethyl phthalate/ethanol in a ratio of 3:1
**Test/control ratio of 3.0 or greater represents a positive result
***Statistically significant difference when compared to the vehicle control group (Group 1) (p<0.05)
****Statistically significant difference when compared to the vehicle control group (Group 1) (p<0.01)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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