[1α(E),2β]-1-(2,6,6-trimethylcyclohex-3-en-1-yl)but-2-en-1-one

Short-term toxicity to fish

Description of key information

Two studies are available for the short-term toxicity of the substance to fish.

Fish acute toxicity was examined in a key study in accordance with OECD TG 203 and in compliance with GLP. In this study 8 Medaka (Oryzias latipes) were exposed to nominal concentrations of 0.49, 0.78, 1.3, 2.0 and 3.2 mg/L test substance under semi-static conditions for 96 hours. The concentrations were analytically verified and geomean measured concentrations were >100% of nominal throughout the test (0.53, 0.84, 1.4, 2.4, 3.7 mg/L, respectively). At a measured concentration of 0.84 mg/L, 25% cumulative mortality was noted and at measured concentration of 1.4 and higher, cumulative mortality was 100%. Based on these data the 96-h LC50 was determined to be 0.97 mg/L (based on measured concentrations and calculated by using binominal test).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

0.97 mg/L

Additional information

The supporting study shows a very similar result. It is an acute toxicity test conducted in compliance with GLP and in accordance with Japanese Industrial Standard (JIS K 0102-1998-71) which is equivalent to OECD TG 203. In this study, 10 medaka fish (Oryzias latipes) per dose level and a control group of 10 fish were exposed to nominal concentrations of 0.151, 0.331, 0.727 and 1.6 mg/L test substance in a semi-static system for 96 hours. Fish were observed for mortality at 24, 48, 72 and 96 hours. Lethal effects were first observed at 0.727 mg/L and 1.6 mg/L. Based on the findings, the 96-hour LC50 value was determined 0.977 mg/L (nominal).