Registration Dossier

Administrative data

Link to relevant study record(s)

Description of key information

Key value for chemical safety assessment

Additional information

There are no in vitro or in vivo data on the toxicokinetics of CyreneTM ((1S,5R)-6,8-dioxabicyclo[3.2.1]octan-4-one).

In the presence of water, CyreneTM, with its anhydrous keto group [C=O] gains water to form the corresponding hydrated geminal diol or Gem Diol [C(OH)2]. An equilibrium is established rapidly and is completely reversible. When the amount of water is reduced, the Gem Diol loses water regenerating anhydrous CyreneTM in its keto form. The ratio of the two forms depends upon the amount of water present and hence the polarity of the solution. Further information is available in the Physical and chemical properties endpoint summary (IUCLID Section 4).

The relevant physiochemical properties are as follows: vapour pressure 28 Pa at 20°C for CyreneTM (measured) and 0.4 Pa at 25°C for Gem Diol (predicted), water solubility of ≥560 g/L for both CyreneTM and Gem Diol (measured) and log Kow of -1.52 at 22°C and pH 5.18 to 5.24 for both CyreneTM and Gem Diol (measured).

Human exposure can occur via the inhalation or dermal routes. Exposure via these routes will largely be to the anhydrous CyreneTM form, although some hydration to Gem Diol is likely in moist air. After absorption, concentrations of the substance in vivo will be low and the Gem Diol will be formed due to the presence of water. The equilibrium between CyreneTM and its Gem Diol form is completely reversible and is established rapidly.

The following summary has therefore been prepared based on validated predictions of the physicochemical properties of the substance itself and the Gem Diol, using this data in algorithms that are the basis of many computer-based physiologically based pharmacokinetic or toxicokinetic (PBTK) prediction models. Although these algorithms provide a numerical value, for the purposes of this summary only qualitative statements or predictions will be made. The main input variable for the majority of these algorithms is log Kow so by using this, and, where appropriate, other known or predicted physicochemical properties of CyreneTM and its Gem Diol form, reasonable predictions or statements may be made about their potential absorption, distribution, metabolism and excretion (ADME) properties.

Absorption

Oral

Significant oral exposure is not expected for this substance.

When oral exposure takes place it can be assumed, except for the most extreme of insoluble substances, that uptake through intestinal walls into the blood occurs. Uptake from intestines can be assumed to be possible for all substances that have appreciable solubility in water or lipid. Other mechanisms by which substances can be absorbed in the gastrointestinal tract include the passage of small water-soluble molecules (molecular weight up to around 200) through aqueous pores or carriage of such molecules across membranes with the bulk passage of water (Renwick, 1993).

Therefore, if oral exposure did occur, the molecular weight of Cyrene™(128.13) is in the favourable range and the water solubility (560 g/L) would favour absorption, so some exposure by this route is likely. Oral exposure is more likely to be to the hydrated Gem Diol form, which also has favourable molecular weight (146.14)and water solubility value (560 g/L) for absorption so exposure to this is also likely.

No signs of systemic toxicity were evident in the acute toxicity (Harlan 2014a) and repeat dose toxicity (Covance 2018b) oral studies.

Dermal

Dermal exposure would, in practice, be principally to the anhydrous form Cyrene™, although exposure to the hydrated Gem Diol is also possible due to the presence of moisture in the air and on the skin surface.

The fat solubility and the potential dermal penetration of a substance can be estimated by using the water solubility- and log Kowvalues. Substances with log Kowvalues between 1 and 4 favour dermal absorption (values between 2 and 3 are optimal) particularly if water solubility is high.

Although both Cyrene™ and the hydrated Gem Diol are highly soluble (560 g/L),the log Kowvalue (-1.52) indicates it that they are unlikely to be sufficiently lipophilic to cross the stratum corneum and therefore dermal absorption into the blood is likely to be minimal.

Inhalation

There is a Quantitative Structure-Property Relationship (QSPR) to estimate the blood:air partition coefficient for human subjects as published by Meulenberg and Vijverberg (2000). The resulting algorithm uses the dimensionless Henry’s Law coefficient and the octanol:air partition coefficient (Koct:air) as independent variables.

Exposure via the inhalation route would be to both the anhydrous form Cyrene™ and to the hydrated Gem Diol due to the presence of moisture in the air and lungs. Using the relevant dimensionless Henry’s Law coefficient values predicts a blood:air partition coefficient of approximately 2.8E+09:1 for the hydrated Gem Diol and approximately 1.75E+05 for Cyrene™ meaning that, if lung exposure occurred, there would be uptake into the systemic circulation for both forms. The water solubility(560 g/L)also suggests that both substances could be dissolved in the mucous of the respiratory tract lining, so may also be passively absorbed from the mucous, further increasing the potential for absorption.

PLACEHOLDER FOR COMMENT ON EVIDENCE FOR SYSTEMIC TOXICITY IN ACUTE TOXICITY (INHALATION) STUDY, IF APPROPRIATE.

 

Distribution

For blood:tissue partitioning a QSPR algorithm has been developed by DeJongh et al. (1997) in which the distribution of compounds between blood and human body tissues as a function of water and lipid content of tissues and the n-octanol:water partition coefficient (Kow) is described. Once absorbed into the blood,concentrations of Cyrene™ in vivo will be low and the Gem Diol will be formed due to the presence of water. A log Kowvalue of -1.52 is relevant for the Gem Diol and indicates that, should systemic exposure occur, potential distribution into the main body compartments would be minimal.

Table: Tissue:blood partition coefficients

 

Log Kow

Kow

Liver

Muscle

Fat

Brain

Kidney

Gem Diol

-1.52

3.02E-02

0.6

0.7

0

0.7

0.8

 

Metabolism

There are no data on the metabolism of Cyrene™ in either the anhydrous or Gem Diol forms.

Genetic toxicity tests in vitro showed no observable differences in effects with and without metabolic activation.

Excretion

Once absorbed into the body,concentrations of Cyrene™ in vivo will be low and the Gem Diol will be formed due to the presence of water. A determinant of the extent of urinary excretion is the soluble fraction in blood. QPSR’s as developed by DeJongh et al. (1997) using log Kowas an input parameter, calculate the solubility in blood based on lipid fractions in the blood assuming that human blood contains 0.7% lipids.

 

Using the algorithm, the soluble fraction ofthe Gem Diol formin blood is >99% meaning that, once absorbed, the substance is likely to be eliminated via the kidneys in urine and accumulation is unlikely.

                                                                                  

 

Renwick A. G. (1993) Data-derived safety factors for the evaluation of food additives and environmental contaminants.Fd. Addit. Contam.10: 275-305.

Meulenberg, C.J. and H.P. Vijverberg, Empirical relations predicting human and rat tissue:air partition coefficients of volatile organic compounds. Toxicol Appl Pharmacol, 2000. 165(3): p. 206-16.

DeJongh, J., H.J. Verhaar, and J.L. Hermens, A quantitative property-property relationship (QPPR) approach to estimate in vitro tissue-blood partition coefficients of organic chemicals in rats and humans. Arch Toxicol, 1997.72(1): p. 17-25.