Registration Dossier

Physical & Chemical properties

Additional physico-chemical information

Administrative data

Endpoint:
additional physico-chemical information
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
Testing was conducted between 14 September 2001 and 4 October 2001.
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of relevant results.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Due to the absence of any analytical verification of the test material concentrations during the ecotoxicology studies the Environment Agency requested additional stability data to show whether the test material concentrations were maintained throughout the duration of the ecotoxicology studies.

Stability trials were therefore performed to assess test material concentration in test media under the conditions of the previously submitted ecotoxicology studies.

The test material concentrations were determined by High Performance Liquid Chromatography (HPLC) using an external standard technique.
GLP compliance:
yes (incl. certificate)
Remarks:
DATE OF INSPECTION: 21 February 2000 DATE OF SIGNATURE: 26 April 2000
Endpoint investigated:
other: Stability in ecotoxicity study media

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): NBPT
- Substance type : Organophosphorus compound
- Physical state : White powder
- Lot/batch No. : C2001-01
- Date received : 19 July 2001
- Stability under test conditions : Determined to be stable over a 96-hour period.
- Storage condition of test material : Approximately 4°C in the dark

Results and discussion

Results:
The substance was found to be stable over a 96-hour period.

Any other information on results incl. tables

The test solutions prepared in dechlorinated tap water adjusted to 160 mg/l CaC03 hardness and enriched algal media (U.S. EPA 1987) stored at 20 °Cand 24 ± 2°C respectively, were determined to be stable over a 96-hour period.

Applicant's summary and conclusion

Conclusions:
The test solutions prepared in dechlorinated tap water adjusted to 160 mg/l CaC03 hardness and enriched algal media (U.S. EPA 1987) stored at 20°Cand 24 ± 2°C respectively, were determined to be stable over a 96-hour period.
Executive summary:

Due to the absence of any analytical verification of the test material concentrations during the ecotoxicology studies the Environment Agency requested additional stability data to show whether the test material concentrations were maintained throughout the duration of the ecotoxicology studies. Stability trials were therefore performed to assess test material concentration in test media under the conditions of the previously submitted ecotoxicology studies.

The test material concentrations were determined by High Performance Liquid Chromatography (HPLC) using an external standard technique.

The test solutions prepared in dechlorinated tap water adjusted to 160 mg/l CaCO3 hardness and enriched algal media (U.S. EPA 1987) stored at 20°Cand 24 ± 2°C respectively, were determined to be stable over a 96-hour period.