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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: studyreport
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Methenamine
EC Number:
202-905-8
EC Name:
Methenamine
Cas Number:
100-97-0
Molecular formula:
C6H12N4
IUPAC Name:
1,3,5,7-tetraazatricyclo[3.3.1.1~3,7~]decane
Details on test material:
not indicated

Test organisms

Test organisms (species):
Nitocra spinipes
Details on test organisms:
Not fed during the study.
Only adult animals , harvested from 3 to 6 weeks old cultures were used.

Study design

Test type:
static
Water media type:
brackish water
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no post exposure period

Test conditions

Hardness:
not indicated
Test temperature:
10 C
pH:
7,8
Dissolved oxygen:
not indicated
Salinity:
0,7%
Nominal and measured concentrations:
at least six concentrations with one control (not specified)
Details on test conditions:
N. spinipes (2x 10 animals) were tested in 15 ml standard laboratory test tubes containing 10 ml of brackish water at 10°C. Mortality was recorded after 96 h under a low power microscope with strong illuminations.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 92 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 26.8 - 305.0
Details on results:
no details given
Results with reference substance (positive control):
no reference substance
Reported statistics and error estimates:
Graphical analysis according to Litchfield and Wilcoxon.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC50 of methenamine in Nitocra spinipes (brackish water) was determined to be 36 g/L.
Executive summary:

The 96 hr-acute toxicity of methenamine to Nitocra spinipes was studied under static conditions. Nitocra were exposed to control and at least 6 test concentrations for 96 hr. Mortality/immobilization were observed at test termination. The 96 hour LC50 was 92.5 g/L.

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with saltwater invertebrates.

Results Synopsis

Test Organism Age: adult

Test Type: Static

LC50: 92.5 g/L (95% C.I. : 26.8 to 305.0 g/L