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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Remarks:
test with Pseudomonas putida
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
Cell multiplication inhibition test
GLP compliance:
no
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Analytical monitoring:
not specified
Details on sampling:
No information available
Vehicle:
yes
Details on test solutions:
Bacterial cultures: Test solutions were prepared in double distilled water in 300 ml Erlenmeyer flasks, stoppered with cotton-lined plastic caps.
Protozoa cultures: Test solutions were prepared in double distilled water in 300 ml Erlenmeyer flasks, stoppered with metal caps.
Test organisms (species):
other: Pseudomonas putida and Entosiphon sulcatum
Details on inoculum:
The flagellate protozoa; Entosiphon sulcatum. The protozoa were fed with E. coli. The test cultures were incubated at 25°C for 72 hours.
The bacterium; Pseudomonas putida. Stock cultures were kept on nutrient medium in agar slant tubes. New stock cultures were prepared weekly. The inoculated stock cultures were incubated at 25°C for 24 hours.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
25°C
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
No information available
Nominal and measured concentrations:
Nominal
Details on test conditions:
Inoculated bacterial test solutions were incubated at 25°C for 16 hours. After termination of the test period the extinction of the monochromatic radiation was measured.
Protozoa cultures were incubated at 25°C for 72 hours, after termination of the test period the cells were counted.
Reference substance (positive control):
not specified
Duration:
72 h
Dose descriptor:
other: Toxicity threshold
Effect conc.:
29 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
growth inhibition
Remarks on result:
other: Entosiphon sulcatum (protozoa)
Duration:
16 h
Dose descriptor:
other: Toxicity Threshold
Remarks:
The toxicological threshold is defined as the 3% differnce against the mean extinction
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Basis for effect:
growth inhibition
Remarks on result:
other: Pseudomonas putida (bacteria)
Details on results:
The 72 hour toxicity threshold of Entosiphon sulcatum to urea was 29 mg/l, and the 16 hour toxicity threshold of urea to Pseudomonas putida was > 10000 mg/l.
Results with reference substance (positive control):
Not examined
Reported statistics and error estimates:
No information available

The 72 hour toxicity threshold of Entosiphon sulcatum to urea was 29 mg/l, and the 16 hour toxicity threshold of urea to Pseudomonas putida was > 10000 mg/l.

Validity criteria fulfilled:
not specified
Conclusions:
The 72 hour toxicity threshold of Entosiphon sulcatum to urea was 29 mg/l, and the 16 hour toxicity threshold of urea to Pseudomonas putida was > 10000 mg/l.
Executive summary:

The 72 hour toxicity threshold of Entosiphon sulcatum to urea was 29 mg/l, and the 16 hour toxicity threshold of urea to Pseudomonas putida was > 10000 mg/l.

Since Entosiphon sulcatum (Euglenophyceae) is not a typical sewage treatment plant organism, the toxicological threshold from Pseudomonas putida was selected for the risk assessment.

Description of key information

The 72 hour toxicity threshold of Entosiphon sulcatum to urea was 29 mg/l, and the 16 hour toxicity threshold of urea to Pseudomonas putida was > 10000 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
10 000 mg/L

Additional information

EC50, LC50, EC10, LC10 or NOEC values are not available, however low toxicity is seen to microorganisms. The 72 hour toxicity threshold of Entosiphon sulcatum to urea was 29 mg/l, and the 16 hour toxicity threshold of urea to Pseudomonas putida was > 10000 mg/l.

Since Entosiphon sulcatum (Euglenophyceae) is not a typical sewage treatment plant organism, the NOEC from

Pseudomonas putida was selected for the risk assessment.