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EC number: 500-107-7 | CAS number: 40039-93-8
The temperature range recorded during the test was 19.3-20.8°C and was within the protocol specified range throughout the test. The average TSS of the activated sludge on the day of collection was 12,687 mg/L. The results of the standard plate count and TSS measurement performed on the inoculum were 1.1 x 106 CFU/mL and 900 mg/L, respectively.
The measured dissolved organic carbon (DOC) on Day 29 and pH values of the test chamber contents on Day 28 are presented in Table 1. The measured concentrations of inorganic carbon in the carbon dioxide trapping solutions are presented in Table 2. The cumulative amounts of CO2 produced over the test period are presented in Table 3. The cumulative percent of theoretical carbon dioxide (% TCO2) evolved is presented in tabular and graphical forms in Table 4 and Figure 1, respectively.
The ready biodegradability of Brominated Epoxy was determined by the Carbon Dioxide Evolution Test Method (OECD Guideline 301B). Tests of ready biodegradability are stringent tests that provide limited opportunity for acclimation and biodegradation to occur. In the CO2 test, inoculated mineral medium was dosed with a known amount of test substance as the nominal sole source of organic carbon and aerated with CO2-free air. The CO2 produced from the mineralization of organic carbon within the test chambers was displaced by the flow of CO2-free air and trapped as K2CO3 in KOH trapping solution. The amount of CO2 produced by the test substance (corrected for that evolved by the blank inoculum) is expressed as a percentage of the theoretical amount of CO2 (ThCO2) that could have been produced if complete biodegradation of the test substance occurred. The test contained a blank control group, a reference group and a treatment group. Each group contained three replicate test chambers. The blank control was used to measure the background CO2 production of the inoculum and was not dosed with a carbon source. The reference chambers were dosed with sodium benzoate, a substance known to be biodegradable, at a nominal concentration of 10 mg C/L. The treatment group test chambers were used to evaluate Brominated Epoxy at a nominal concentration of 10 mg C/L. The results indicated that the activated sludge inoculum was active, degrading the reference substance an average of 101.8%. The average cumulative percent biodegradation for Brominated Epoxy was –2.4%.
Biodegradation for Brominated Epoxy
Evidence of ready biodegradability in a Carbon Dioxide Evolution Test is 60% ThCO2 within the 28-day test period. In addition, the pass level must be reached within 10 days of achieving 10% ThCO2. The final mean percent biodegradation for Brominated Epoxy was –2.4%. Brominated Epoxy may not be considered readily biodegradable since the pass level of 60% ThCO2 was not achieved.
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