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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: non-GLP, guideline study, fully adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
not specified
Test type:
fixed dose procedure
Limit test:
yes

Test material

Constituent 1
Reference substance name:
Reference substance 001
Cas Number:
11071-47-9
Molecular formula:
C8H16
Constituent 2
Reference substance name:
Di-n-butene
IUPAC Name:
Di-n-butene
Details on test material:
Di-n-butene. According to the report's owners the substance di-n-butene can be classified as CAS 11071-47-9 (Isooctene).
Components: 18 % n-Octene, 58 % Methylheptene and 24 % Dimethylhexene

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: SPF TNO
- Weight at study initiation: 118.6 g
- Fasting period before study: 16 hours
- Housing: stainless steel cages
- Diet (e.g. ad libitum): R10 Alleindiat for rats
- Water (e.g. ad libitum): tapwater
- Acclimation period: 4-8 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 1
- Humidity (%): 60 ± 5
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12 /12

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Doses:
13699 cm3/kg
No. of animals per sex per dose:
5 per sex (total 10)
Control animals:
no
Details on study design:
Animals were randomly allocated to stainless steel cages

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations at 6 hours after dosing and daily. Animals were weighed at 1, 7 and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: necropsy with macroscopic examination
Statistics:
LC50 calculated following Litchfield and Wilcoxon method with 95% confidence intervals

Results and discussion

Effect levels
Dose descriptor:
LD50
Effect level:
> 10 000 mg/kg bw
Mortality:
None
Clinical signs:
other: After one hour the animals exhibited piloerection. Then animals crouched, had slight tremors, diuresis, lightly coloured faeces with a strong smell of the test substance. After 48 hours the animals were free of these clinical signs.
Gross pathology:
One animal had partial thickening of the forestomach and another partial hyperemia of the small intestine membrane.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Remarks:
Migrated information
Conclusions:
The LD50 of di-n-butene in rats was >10,000 mg/kg. Animals were free of clinical signs after 48 hours. At necropsy, one animal had partial thickening of the forestomach and another partial hyperemia of the small intestine membrane.