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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Remarks:
limited documentation, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: AFNOR NF T90-376 Qualité de l'eau - Détermination de la toxicité chronique vis-à-vis de Ceriodaphnia dubia en 7 jours - Essai d'inhibition de la croissance de la population
Version / remarks:
draft
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Phenanthrene
EC Number:
201-581-5
EC Name:
Phenanthrene
Cas Number:
85-01-8
Molecular formula:
C14H10
IUPAC Name:
phenanthrene
Test material form:
solid
Details on test material:
- Substance type: organic
- for additional information see study record
Specific details on test material used for the study:
- Name of test material (as cited in study report): phenanthrene
- Source of test material: no data
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
no data

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution close to water saturation used for dilution series (no detailed data, see Report p. 34)
- Eluate: no
- Differential loading: no
- Controls: with and without vehicle
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no data

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Source: INERIS
- Age of parental stock (mean and range, SD): < 24 h
- Feeding during test
- Food type: Chlorella vulgaris / Pseudokirchneriella subcapitata / fish diet
- Offspring: Males were counted and removed from the test medium.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d

Test conditions

Hardness:
240 mg/L (as CaCO3)
Test temperature:
25 ± 2 °C
pH:
8.1 ± 0.4
Dissolved oxygen:
> 7.0 mg/L
Nominal and measured concentrations:
Nominal and analytical values not documented.
Analytical: calculation of the geometric mean from measured concentrations in fresh and spent medium, based on an arithmetic model (OECD 1999) (see Report, p. 44/45)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed (screw cap)
- Material, size, headspace, fill volume: glass vessel (90 mL), test volume 50 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): 1x/d, 5x/wk (each workday)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): none

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural mineral water (Evian)
- Ca/Mg ratio: approx. 3:1
- Conductivity: 460 ± 100 µs/cm

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 500 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality adults and neonates, abortions, reproduction

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: no data
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
13 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
other: mortality and reproduction
Key result
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
15 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality and reproduction
Remarks on result:
other: 95% C.I.: 4.7 - 19 µg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In a reproduction inhibition study according to test guideline AFNOR NF T90-367 ("Inhibition of population growth") with Ceriodaphnia dubia, an EC10 of 13 µg/L was determined (measured concentrations - geometric mean). Exposure was for seven days in closed test vessels under semi-static conditions.