Registration Dossier

Administrative data

Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Determination of the Adsorption Coefficient (OECD 121} of the Test ltem E12MNA Oxazolidine (Sa 190}
Deviations:
no
Principles of method if other than guideline:
HPLC is performed on analytical columns packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix is used. While passing through the column along with the mobile phase the test substance interacts with the stationary phase. As a result of partitioning between mobile and stationary phases the test substance is retarded. The retention time of the test substance is evaluated by setting up a calibration plot for reference substances of known adsorption coefficient. By applying the regression equation of the calibration plot, the adsorption coefficient of the test substance can be calculated.

The adsorption coefficient Koc is defined as the ratio between the concentration of the substance in the soil/sludge and the concentration of the substance in the aqueous phase at adsorption equilibrium. The experimental method described in OECD Guideline 121 uses HPLC for the estimation of the adsorption coefficient in soil and sewage sludge.
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Radiolabelling:
no

Study design

Analytical monitoring:
yes

Results and discussion

Transformation products:
not measured
Dissipation DT50 of parent compoundopen allclose all
pH:
7
Temp.:
20 °C
Hydrolysis rate constant:
ca. 0.006 h-1
DT50:
ca. 108 h
pH:
7
Temp.:
50 °C
Hydrolysis rate constant:
ca. 0.018 h-1
DT50:
ca. 38 h
pH:
7
Temp.:
70 °C
Hydrolysis rate constant:
ca. 0.143 h-1
DT50:
ca. 5 h
Other kinetic parameters:
A retention time of 4.040 min was determined for the test item. This is higher than the retention time for the reference item phenanthrene, thus, log Koc can be reported as:
log Koc > 4.09
Details on results:
A retention time of 4.040 min was determined for the test item. This is higher than the retention time for the reference item phenanthrene, thus, log Koc can be reported as:
log Koc > 4.09

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A retention time of 4.040 min was determined for the test item. This is higher than the retention time for the reference item phenanthrene, thus, log Koc can be reported as:
log Koc > 4.09
Executive summary:

A retention time of 4.040 min was determined for the test item. This is higher than the retention time for the reference item phenanthrene, thus, log Koc can be reported as:

log Koc > 4.09