Registration Dossier
Registration Dossier
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EC number: 215-609-9 | CAS number: 1333-86-4
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- In vitro toxicity of carbon nanotubes, nano-graphite and carbon black, similar impacts of acid functionalization
- Author:
- Figarol A, Pourchez J, Boudard D, Forest V, Akono C, Tulliani JM, Lecompte JP, Cottier M, Bernache-Assolant D, Grosseau Ph
- Year:
- 2 015
- Bibliographic source:
- Toxicology in Vitro 30(1 Part B), pp.476-485.
Materials and methods
- Type of study / information:
- Cytotoxicity
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The in vitro cytotoxicity of pristine and acid-treated carbon black was assessed in the RAW 264.7 murine macrophage cell line (24 hours exposure). LDH release was used for cytotoxicity assessment. The production of the cytokine TNF-α (tumour necrosis factor α) informed about the pro-inflammatory activity after a 24 h. ROS production was measured after 90 min of exposure.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Carbon black
- EC Number:
- 215-609-9
- EC Name:
- Carbon black
- Cas Number:
- 1333-86-4
- Molecular formula:
- C
- IUPAC Name:
- carbon
- Test material form:
- solid: nanoform, surface-treated
Constituent 1
Results and discussion
Any other information on results incl. tables
Acid functionalization had no impact on LDH release and TNFalpha production. The level of ROS production was reduced after acid treatement of carbon black.
Applicant's summary and conclusion
- Conclusions:
- Acid treatment of carbon black induced no cytotoxic or pro-inflammatory responses in a murine macrophage cell line. These data do not support an increased cytotoxicity of carbonyl-functionalised carbon blacks.
- Executive summary:
Acid functionalization had no impact on LDH release and TNFalpha production in a murine macrophage cell line. The level of ROS production was reduced after acid treatement of carbon black.
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