Allyl alcohol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Peer reviewed publication. Method used in study is similar to OECD 401, but this study predates GLP and individual animal data are not available.

Data source

Reference

Reference Type:

publication

Title:

The toxicity of allyl alcohol.

Author:

Dunlap, M.K., Kodama, J.K., Wellington, J.S., Anderson, H.H., Hine, C.H.

Year:

1958

Bibliographic source:

AMA Archives of Industrial Health. 18:303-311

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

individual animal responses not given, males only

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Allyl alcohol

EC Number:

203-470-7

EC Name:

Allyl alcohol

Cas Number:

107-18-6

Molecular formula:

C3H6O

IUPAC Name:

prop-2-en-1-ol

Details on test material:

Allyl alcohol provided by the Shell Chemical Company
Purity: 98.5% (Impurities are diallyl ether and water)

Test animals

Species:

rat

Strain:

Long-Evans

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 111 - 143 grams and 170 - 252 grams.
- Housing: groups of 5-6 in one cage
- Diet (e.g. ad libitum): standard pellets ad libitum
- Water (e.g. ad libitum): ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: distilled water

Details on oral exposure:

Intragastric administration using ball-point needle and syringe to introduce fluid into the stomach.

Doses:

Various doses (unspecified) of allyl alcohol, 1% in distilled water, were given to groups of 5 male rats.

No. of animals per sex per dose:

5 males rats per dose.

Control animals:

no

Details on study design:

- Duration of observation period following administration: not less than 10 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, histopathology, gross pathology

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

105 mg/kg bw

Based on:

test mat.

Remarks on result:

other: For animals weighing 111-143 g

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

99 mg/kg bw

Based on:

test mat.

Remarks on result:

other: For animals weighing 170-252 g

Mortality:

No details

Clinical signs:

other: The main clinical sign was described as apathy along with anxiety. Lacrimation and tremors were noted in some rats. Coma and diarrhoea preceded death in moribund animals.

Gross pathology:

Oedema and congestion of the lungs, visceral congestion, presence of mucous in the intestinal tract, discoloured liver (some necrosis), swollen discoloured kidneys.

Other findings:

Histopathological examination showed lung congestion, liver damage (congestion and necrosis of periportal sinusoids, central pallor and necrosis), presence of heme casts and a cloudy swelling in the kidney. Rats surviving the 10 day observation period showed similar lesions to rats that had died, but less frequently.

Any other information on results incl. tables

The LD50 values were calculated according to Weil's modification according to the method of Thompson. 95% confidence limits were not reported.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Under the conditions of this study the LD50 of allyl alcohol is between 99 - 105 mg/kg bw in rats.

Executive summary:

In an acute oral toxicity study, groups of 5 male long-evan rats were given a single oral dose of graded quantities of allyl alcohol solution and observed for 10 days.

 

Oral LD₅₀Males =  99 - 105 mg/kg bw