4H-1,2,4-Triazole, 4-[[bis(4-fluorophenyl)methylsilyl]methyl]-

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: U.S. EPA Pesticide Assessment Guidelines Subdivision F, 84-2

Deviations:

no

Remarks:

Conducted according to guideline in effect at time of study conduct

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

bis(4-fluorophenyl)methyl(4H-1,2,4-triazol-4yl-methyl)silane

EC Number:

600-198-4

Cas Number:

101377-47-3

Molecular formula:

C16H15N3F2Si

IUPAC Name:

bis(4-fluorophenyl)methyl(4H-1,2,4-triazol-4yl-methyl)silane

Details on test material:

- Purity: 95%

Method

Target gene:

histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA1537, TA97, TA98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254-induced rat liver S9

Test concentrations with justification for top dose:

Without activation: 0, 5, 10, 50, 100, 500, 750 µg/plate
With activation: 0, 10, 50, 100, 500, 1000, 2500 µg/plate

Controls

Untreated negative controls:

yes

Remarks:

DMSO

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene(all strains +S9), N-methyl-N'-nitro-N-nitrosoguanidine (TA1535 and TA100 -S9), 9-aminoacridine (TA97), 2-nitrofluorene (TA98)

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA1537, TA97, TA98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Except for strain TA98 in the presence of an activation system, no significant increases in revertants, compared with controls, were observed. In trial 1 with strain TA98 in the presence of an activation system, a significant (p<0.01) increase in revertants, compared to controls, was indicated at 1000 µg/plate. The probability of a positive linear dose response was 0.0041. In trial 2, significant increases in revertants were indicated at 50 and 5000 µg/plate. However, there was no positive linear dose response. Therefore, the results of Trial 2 indicated a nonpositive mutagenic response. A third trial was performed. No significant increases in revertants were indicated. This test substance was judged to be not mutagenic in strain TA98 in the presence of an activation system based on the results of nonpositive mutagenic responses in two of three trials.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The test substance was nonmutagenic when tested in Salmonella typhimurium strains TA1535, TA97, TA98 and TA100 in the absence and presence of S9.

Executive summary:

The test substance was nonmutagenic when tested in Salmonella typhimurium strains TA1535, TA97, TA98 and TA100 in the absence and presence of an exogenous metabolic activation system (S9). The maximum concentration tested was 750 µg/plate without activation and 2500 µg/plate with activation. Statistical analyses were performed on the number of revertants obtained for each strain in two trials. Except for strain TA98 in the presence of an activation system, no significant increases in revertants, compared with controls, were observed. A third trial was conducted with strain TA 98 in the presence of an activation system. The test substance was judged to be not mutagenic in strain TA98 in the presence of an activation system based on the results of nonpositive mutagen responses in two of the three trials.