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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Qualifier:
according to guideline
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Sampling method: t=0, t=48hr
- Sample storage conditions before analysis: 2-10C and shpped at end of study
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
In a sparging system, the test material was purged through test medium for different periods at a rate of approximately 20 mL er minute. The gas flow rate was assesseed using a soap- bubble flow meter. Air was simultaneoulsy purged to maintain acceptable levels of dissolved oxygen.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: in house laboratory culture with known history
young daphnia less than 24 hours old from parental daphnids older than 2 weeks of age
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
pH:
start of test pH 7.9 - 8.0
48 hrs - pH 7.8 - 8.0
Dissolved oxygen:
start of test 5.7 - 9.6
48 hr 6.0 -9.0
Nominal and measured concentrations:
nominal concentration - number of minutes of purging test material at 20 mL/min
measured - below limit except at 60 minutes purging resulting in a measured concentration of 83 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type :closed
- Material, size, headspace, fill volume: 100 ml, all glass, filled up to rim
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per vehicle control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours

Reference substance (positive control):
yes
Remarks:
K2Cr2O7
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
83 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: none

- Other biological observations: none
- Mortality of control:none
- Other adverse effects control:none
- Abnormal responses: none
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Mortality: yes
- EC50/LC50: 48 hr - 0.51 mg/L
Validity criteria fulfilled:
yes
Remarks:
no daphnia immobilized in control, oxygen concentration above 5 mg/L at end of test, temperature and pH maintained, potassium dichromate results within acceptable range
Conclusions:
Under the conditions of the present test, the 48 hr EC50 for daphnia magna exposed to the test substance was > 83 mg/L. Since no effect was recorded at this level, it can be safely concluded that the 48 hr EC50 for the test substance is > 100 mg/L and the test substance can be classified as not harmful for crustaceans.

Description of key information

HFO-1234yf has low toxicity to daphnia

Key value for chemical safety assessment

Additional information

HFO-1234yf has low toxicity to daphnia magna with an EC50 greater than 100 mg/l.