Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 468-710-7 | CAS number: 754-12-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to fish
Administrative data
- Endpoint:
- fish, juvenile growth test
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The dissolved oxygen concentrations were not maintained at 70% or more of the air saturation value throughout the test, this was a consequence of testing a gas in air-tight closed vessels. However, no negative effects were observed.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 215 (Fish, Juvenile Growth Test)
- Version / remarks:
- 2000
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.14 (Fish Juvenile Growth Test)
- Version / remarks:
- 2008
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 468-710-7
- EC Name:
- -
- Cas Number:
- 754-12-1
- Molecular formula:
- C3H2F4
- IUPAC Name:
- 2,3,3,3-tetrafluoroprop-1-ene
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All test concentrations and stock solution
- Sampling method: The samples were taken in a 20 ml GC-vial that already contained 0.5 ml acetone.
- Sample storage conditions before analysis: none
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of stock solutions by loading two 10 L gas sampling bags each with 7 or 7.5 litres of ISO medium followed by addition of ~3.5 or 3.75 grams gas (500 mg/l). The gas sampling bags were closed immediately after addition and then shaken carefully for 4 hours on a platform shaker. Subsequently, the bags were left to settle overnight. The two saturated media (expected concentration ~160 mg/l) were mixed and then used for preparing the test concentrations by serial dilution.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
- Weight at study initiation (mean): 0.76 ± 0.08 g
FEEDING DURING TEST
- Food type: Cyprico Crumble Excellent (300-500 um), Coppens International bv, Helmond, The Netherlands).
- Amount: 4% of body weight per vessel
- Frequency: daily
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- 211.5 mg/L
- Test temperature:
- 20.0 - 21.6 °C
- pH:
- 6.9 - 7.9
- Dissolved oxygen:
- In freshly prepared solutions: 8.3 - 10.1 mg/L
In 24 h old solutions: 2.1 - 6.2 mg/L
In 24 h old control: 1.8 - 6.2 mg/L - Nominal and measured concentrations:
- Nominal test substance concentrations: 1.5, 3.0, 6.0, 12 and 24 mg/L
Mean measured substance concentrations: 1.2, 2.7, 5.4, 11 and 22 mg/L (82 - 91% of nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: airthight glass vessels (40 L)
- Type: closed
- Fill volume: 38 L
- Aeration: none
- Renewal rate of test solution (frequency): daily
- No. of organisms per vessel: 16
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.32 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium, formulated using RO-water (tapwater purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daily determination of mortalities, clinical sings were determined three times a week, body weight was determined at test start and after 14 and 28 days of exposure respectively. - Reference substance (positive control):
- yes
- Remarks:
- penta-chlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 22 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 5.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Type of and number with morphological abnormalities: none
- Type of and number with behavioural abnormalities: none
- Other biological observations: no mortalities observed
Statistical analysis on the growth rates for the interval of days 0-14 showed that the growth rate of the fish exposed to test groups of 5.4 and 22 mg/l were statistically significant lower than the growth of the fish in the control group. - Results with reference substance (positive control):
- The reference test showed normal sensitivity of the supplied carp.
- Reported statistics and error estimates:
- ANOVA, Tukey test, Bonferroni t-test, TOXSTAT Release 3.5, 1996, D.D. Gulley, A.M. Boelter, H.L. Bergman
Any other information on results incl. tables
Table 1: Mean fish weights recorded at the start, after 14 and 28 days.
Day of recording |
Mean fish weights (g): |
|||||
Control |
1.2 mg/L |
2.7 mg/L |
5.4 mg/L |
11 mg/L |
22 mg/L |
|
0 |
0.76 |
0.79 |
0.78 |
0.77 |
0.78 |
0.73 |
14 |
1.11 |
1.11 |
1.10 |
1.06 |
1.10 |
1.01 |
28 |
1.49 |
1.48 |
1.49 |
1.40 |
1.45 |
1.27 |
% increase on day 28* |
196 |
189 |
191 |
182 |
186 |
174 |
*Based on non-rounded figures.
Table 2: Mean growth rates recorded for the intervals of 0-14 days and 0-28 days.
Interval |
Mean growth rates: |
|||||
Control |
1.2 mg/L |
2.7 mg/L |
5.4 mg/L |
11 mg/L |
22 mg/L |
|
Day 0-14 |
2.71 |
2.45 |
2.48 |
2.22 |
2.47 |
2.32 |
Day 0-28 |
2.42 |
2.28 |
2.32 |
2.11 |
2.20 |
1.96 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.