Registration Dossier

Ecotoxicological information

Toxicity to other above-ground organisms

Administrative data

Endpoint:
toxicity to other above-ground organisms
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Even if the species is not currently used in the standardised test, the test conditions are detailed and scientifically acceptable. The GLP are not mentioned.

Data source

Reference
Reference Type:
publication
Title:
Acute toxicity of substituted phenols to Rana japonica tadpoles and mechanisms-based quantitative structure-activity relationship (QSAR) study
Author:
Wang X, Dong Y, Wang L, Han S
Year:
2001
Bibliographic source:
Chemosphere, 44, 447-455.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Deviations:
not applicable
Principles of method if other than guideline:
Investigation of the toxicity of substances on frog tadpoles in a 24h  exposure test.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
provided by Department of Chemistry at Nanjing University (analytical purity).

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
PREPARATION AND APPLICATION OF TEST SOLUTION:
Method: Aerated chloride-free water was used for preparing the stock solutions of chemicals and test dilution.
Control: yes, the aerated chloride-free water without test compounds served as the control. 
Evidence of undissolved material: no data.

Test organisms

Test organisms (species):
other: Rana Japonica tadpoles
Details on test organisms:
TEST ORGANISM
- Common name: Japanese Brown Frog.
- Strain: no data
- Source: naturally fertilized egg masses collected from a permanent pond  in Nanjing.
- Age at study initiation: 30-day old.
- Weight at study initiation: 0.09 (+/-) 0.01 g
- Method of culturing: in aerated stream water in the laboratory at room  temperature (22-25°C).
- Feeding during test: no, feeding was halted 24h prior to testing.
ACCLIMATION
- Acclimation period: no acclimatation.

Study design

Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
none

Test conditions

Details on test conditions:
STUDY DESIGN
- Test type: semi-static.
- Test vessel type: open.
- Water media type: fresh.

TEST CONDITIONS:
- Hardness:data not available.
- Test temperature: 22-25°C.
- pH: 6.63
- Dissolved oxygen: data not available.
- Nominal and measured concentrations: no data

Details on test conditions:
TEST SYSTEM
- Test vessel: 1000-mL pyrex beaker containing 500 mL test solution.
- Renewal rate of test solution: every 6 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control / vehicle control: (replicates): 3

TEST MEDIUM / WATER PARAMETERS: aerated chloride-free water. No other information.

OTHER TEST CONDITIONS
- Adjustment of pH: no.
- Photoperiod: 14h light, 10h dark.
- Light intensity: data not available.

EFFECT PARAMETERS MEASURED: mortality.

STATISTICAL ANALYSIS: using non-linear regression in Statistica for Window software (1995).

Nominal and measured concentrations:
- Spacing factor for test concentrations: a six-step concentration series  in geometric grade ranging from no effect to 100% lethal concentration  
were set after range finding experiments.  
RANGE-FINDING STUDY: yes.
- Results used to determine the conditions for the definitive study: 6  concentrations between 0 and 
100% lethality.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
12 h
Dose descriptor:
LC50
Effect conc.:
2.371 other: mmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
2.371 other: mmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No mortality occurred in the control. 

Any other information on results incl. tables

  The result can be converted to 24h LC50 = 294 mg /L.

Applicant's summary and conclusion