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Four data are referenced for terrestrial toxicity:

- toxicity to soil macroorganisms: Hartenstein (1982, reliability 3) measured the growth rate and mortality of Eisenia foetida after 2 weeks of exposure with the test substance in activated sludge. A 42 day LC100= 40 g/kg soil dry weight (4% w/w reported in the publication) was calculated based on nominal test material concentration, for mortality effect, and a 42 day LOEC= 10 g/kg dry weight (1% w/w reported) was calculated based on nominal test material concentration, for growth rate.

- toxicity to terrestrial plants: Wang et al. in 2002 (reliability 3) measured by a filter paper contact method the seed germination of Cucumis sativus when PMP is applicated for 2 days. The resulting 2 day EC50 was 229.3 mg/L based on nominal test material concentration.

- toxicity to birds: Schafer and Jacobson in 1983 (reliability 3) carried out an acute oral toxicity on Agelaius phoeniceus (red- winged blackbird). The measured LD50 was > 113 mg/kg bw.

- toxicity to frogs: Wang et al. in 2001 (reliability 2) investigated the PMP toxicity on frog tadpoles. The study was conducted in semi static conditions, in freshwater, the exposure duration was 24 hours. No mortality occurred in the control. The resullting 24h LC50 was 294 mg/L.

Additional terrestrial tests are not required since mequinol is not supposed to be directly applied to soil and an indirect exposure to soil via sewage sludge transfer is unlikely since the substance is readily biodegradable. For such a substance, it can be assumed that it will be biodegraded within the STP process and as a consequence a transfer to the soil compartment is not expected. Furthermore, since Mequinol is neither adsorptive (log Koc = 1,74) nor bioaccumulative (log Kow = 1,3), a significant distribution into the soil compartment and a significant exposure of terrestrial organisms is not expected. Hence, information about effects on terrestrial organisms is not required and the equilibrium partitioning method is used for assessing the hazard to terrestrial organisms.