2-[2-(2-ethoxyethoxy)ethoxy]ethyl methacrylate

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented study report which meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Determination of in vitro hydrolysis rates of methacrylate esters; determination of half-lifes in rat liver microsomes and whole rat blood.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-[2-(2-ethoxyethoxy)ethoxy]ethyl methacrylate

EC Number:

254-588-0

EC Name:

2-[2-(2-ethoxyethoxy)ethoxy]ethyl methacrylate

Cas Number:

39670-09-2

Molecular formula:

C12H22O5

IUPAC Name:

2-[2-(2-ethoxyethoxy)ethoxy]ethyl methacrylate

Constituent 2

Reference substance name:

2-(2-(2-ethoxy ethoxy)-ethoxyethyl methacrylate

IUPAC Name:

2-(2-(2-ethoxy ethoxy)-ethoxyethyl methacrylate

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): ethoxytriethylene glycol methacrylate

Radiolabelling:

no

Test animals

Species:

other: rat liver microsomes and rat blood

Administration / exposure

Vehicle:

DMSO

Duration and frequency of treatment / exposure:

120 min (samples collected at 0, 2, 5, 15, 30, 60 and 120 minutes)

Doses / concentrations

Remarks:

Doses / Concentrations:
0.25 mM

No. of animals per sex per dose / concentration:

not applicable; in vitro test

Control animals:

other: not applicable; in vitro test

Positive control reference chemical:

Methyl methacrylate

Details on dosing and sampling:

METABOLITE CHARACTERISATION STUDIES
- Method type(s) for identification: liquid chromatography separation with accurate mass quadrupole/time-of-flight mass spectrometry detection (LC/QTOF-MS) to quantitate methacrylic acid concentrations
- Limits of detection and quantification: LLQ = 0.0117 mM methacrylic acid

Results and discussion

Main ADME results

Type:

metabolism

Results:

the ester was rapidly converted to MAA in whole rat blood and rat liver microsomes: half life 4.95 min (liver microsomes) / 27.72 min (blood)

Any other information on results incl. tables

Negative controls in the rat liver microsome experiments included incubations with heat-inactivated microsomes, no microsomes and no NADPH. Removal of NADPH made little or no difference in hydrolysis rates. Heat inactivation significantly reduced hydrolysis rates, and absence of microsomes resulted in no hydrolysis. 

ET3EGMA was rapidly converted to MAA in whole rat blood and rat liver microsomes with hydrolysis half-lives of 4.95 min (liver microsomes) and 27.72 min (blood).

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): other: The metabolism data show that ET3EGMA is rapidly hydrolysed in vitro.
The metabolism data show that ET3EGMA is rapidly hydrolysed in vitro.

Executive summary:

This in vitro metabolism study was conducted to investigate in vitro hydrolysis rates of ET3EGMA. Half-lifes were determined in rat liver microsomes and whole rat blood.

ET3EGMA was rapidly converted to MAA in whole rat blood and rat liver microsomes with hydrolysis half lives of 4.95 min (liver microsomes) and 27.72 min (whole blood)