4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline 201 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

EC Number:

500-070-7

EC Name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Cas Number:

30583-72-3

Molecular formula:

(C15H28O2.C3H5ClO)x

IUPAC Name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Constituent 2

Reference substance name:

Cyclohexanol, 4,4'-(1-methylethylidene)bis-, polymer with 2-(chloromethyl)oxirane

IUPAC Name:

Cyclohexanol, 4,4'-(1-methylethylidene)bis-, polymer with 2-(chloromethyl)oxirane

Details on test material:

As per IUCLID Sections 1.1. 1.2. and 4.1.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The appropriate amount of test substance was added to 250 ml glass flasks to yield a concentration of approximately 100 mg/L when mixed with alagal stock culture growth medium for one hour.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Algal cultures were grown in Algal stock culture medium (Woods Hole) with up to 300 mg EDTA/L by Stillmeadow. The cultures were maintained at 24 =/- 2 degrees C with constant aeration and continuos lighting of 400 +/- 40 ft.c.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

None

Test conditions

Hardness:

No data

Test temperature:

23 C

pH:

7.8 mean

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

100 mg/L nominal (Limit Test)

Details on test conditions:

Cultures were prepared to contain approximately 50,000 algal cells/ml. There were six flasks for treated cultures, five flasks for the untreated control and three flasks for the positive control 10 mg/ml zinc chloride. All flasks were set on a shaker table for constant shaking at approximately 100 cycles/minute. At interval of 24, 48 and 72 hours following the intiation of treatment all cultures were sampled to assess cell density with a hemacytometer.

Reference substance (positive control):

yes

Remarks:

Zinc chloride at 10 mg/L

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Details on results:

The mean relative percent survial of the 100 mg/L Limit Dose after 72 hour of treatment was 108% with terminal pH of 7.6. Control culture mean pH was 7.7.

Results with reference substance (positive control):

No algal cell growth was observed in the positive control cultures.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Mean control cell density increased by a factor > 16.

Conclusions:

The EC50 and NOEC for the test substance to green algae is > 100 mg/L.

Executive summary:

The EC50 and NOEC for the test substance, 4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, to green algae in an O.E.C.D. test guideline 201 study is > 100 mg/L.