4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

EC Number:

500-070-7

EC Name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Cas Number:

30583-72-3

Molecular formula:

(C15H28O2.C3H5ClO)x

IUPAC Name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Constituent 2

Reference substance name:

Cyclohexanol, 4,4'-(1-methylethylidene)bis-, polymer with 2-(chloromethyl)oxirane

IUPAC Name:

Cyclohexanol, 4,4'-(1-methylethylidene)bis-, polymer with 2-(chloromethyl)oxirane

Details on test material:

As per Sections 1.1. 1.2 and 4.1. .

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

The activated sludge was acquired from the Brazos River Valley Sewer Authority and maintained wit aeration before use.

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Ten BOD bottles were prepared for the blank control, 5 mg/L test substance and reference solutions for up to five sampling intervals. the BOD bottles were stored in the dark and incubatedat 21-22 degrees C. Two BOD bottles for each solution were removed at each sampling intervak of 0, 7, 14, 21 and 28 days. The oxygen in each duplicate set of solutions was determined with a YSI Model 5100 Dissolved Oxygen Metter. The Chemical Oxygen Demand of the test substance was determined to be 2.281 mg O2/mg.

Reference substance

Reference substance:

aniline

Remarks:

2.0 mg/L

Results and discussion

Test performance:

As per the O.E.C.D. test guideline.

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

ca. 0.1

Sampling time:

28 d

Details on results:

The mean percent biodegradation of the test substance over the 28-day contact period was 0.1%.

BOD5 / COD results

Results with reference substance:

The Reference Substance aniline (2 mg/L) was biodegraded 83.1% by day 14 and 98.6% by day 28.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance was not biodegradable under the conditions of the study.

Executive summary:

The test substance, 4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, was not biodegradable under the conditions of the study.