Registration Dossier

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Old publication, not according to any guidelines, insufficient description of the method.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1969
Report Date:
1969

Materials and methods

Principles of method if other than guideline:
other: BASF-test
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity: >= 99.9 %Molecular weight: 126.13pH (aqueous solution): 7.5 - 8.0Boiling point: Sublimation at > 300 °CVapour pressure (20 °C): < 10-5 TorrFlammability: 354 °C

Test animals

Species:
mouse
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: Traganth
Doses:
200, 400, 800, 1000, 1250, 1600 mg/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
Surviving animals were observed for one week after exposure and then sacrificed. A post mortem examination was performed.

Results and discussion

Effect levels
Dose descriptor:
LD50
Effect level:
800 mg/kg bw
Mortality:
1600 mg/kg: All animals died within 7 days after the administration.
1250 mg/kg: 9/10 animals died within 48 hours after the administration.
1000 mg/kg: All animals died within 48 hours after the administration.
800 mg/kg: 4/10 animals died within 7 days after the administration.
400 mg/kg: All animals survived until the end of the study.
200 mg/kg: 1/10 animals died within 24 hours after the administration.
Clinical signs:
Immediately after the administration in all animals accelerated breathing, tumbling, retracted flanks, raised fur, closed eyes. On the consecutive days huddled posture, face-down position, apathy. Recovery of the survivors 3 - 5 days after the administration.
Gross pathology:
deceased animals: intestinal tract, atonic ; sacrificed animals: liver, adhesions

Applicant's summary and conclusion

Conclusions:
LD50 = 800 mg/kg