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Toxicological information

Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Scientific publication, the reliability can not be judged. A new test system for predicting carcinogenicity.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
In vitro determination of carcinogenicity of sixty-four compounds using a bovine papilloma virus DNA-carrying C3H/10T(1/2) cell line.
Author:
Kowalski LA et al.
Year:
2000
Bibliographic source:
Environ. Mol. Mutagen. Vol. 35(4), 300-311
Reference Type:
secondary source
Title:
Unnamed
Year:
2009

Materials and methods

Type of study / information:
In vitro test for predicting rodent carcinogenicity
Principles of method if other than guideline:
A new test system for predicting carcinogenicity was tested with 64 chemicals.

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Melamine was purchased from Sigma-Aldrich Chemicals.

Results and discussion

Any other information on results incl. tables

The response of the T1 cells to the test substance melamine was positive and the lowest dose resulting in transformation of the cells was 10 ng/ml.

Applicant's summary and conclusion

Executive summary:

The authors developed a new in vitro early-stage assay for testing both genotoxic and nongenotoxic carcinogens, and tested ca. 160 substances.
The test system used is based on a mouse cell line that carries bovine papilloma virus type 1 DNA (T1 assay).

The response of the T1 cells to the test substance melamine was positive and the lowest dose resulting in transformation of the cells was 10 ng/ml.