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Ecotoxicological information

Long-term toxicity to fish

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Reference
Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: study design based on national standard method; detailed documentation (also assessed by OECD)
Qualifier:
according to
Guideline:
other: see below
Principles of method if other than guideline:
Method used is based on US-EPA procedure 'Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians; EPA-660/3-75-009 (1975)
GLP compliance:
no
Specific details on test material used for the study:
TPP purchased from Eastman Kodak Comp., no further information
Analytical monitoring:
yes
Details on sampling:
Water samples were taken for analysis
Vehicle:
yes
Details on test solutions:
Acetone - no concentrations given
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Rainbow trout sac-fry 10 days old, average weight 0.081 g and average length: 22.3 mm were used. Acclimatisation took place over about 4 days
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 d
Hardness:
295 - 305 mg/L as CaCO3
Test temperature:
12 °C
pH:
7.4 - 7.5
Dissolved oxygen:
7.3 - 8.5 mg/L
Salinity:
Not applicable
Nominal and measured concentrations:
0.055, 0.090, 0.125, 0.16, 0.24, 0.31, 0.45 mg/L (nominal); highest concentration measured and used for estimation of the lower concentrations
Details on test conditions:
Glass aquaria (30 x 60 x 30cm) divided into two small equal and one large chamber were used, total volume of test solution maintained at about 42l, connected to a dilution device to give measured concentrations of TPP. 30 fish used for each test.
Reference substance (positive control):
no
Duration:
30 d
Dose descriptor:
LOEC
Effect conc.:
0.055 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
30 d
Dose descriptor:
EC10
Effect conc.:
0.037 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
EC10 based on test with sac fry
NOEC based on test with fingerling under same conditions
Conclusions:
In a flow through study a 30 day LOEC of 0.055 mg/L was determined for the sac fry stage regarding the endpoints length and weight. From the test raw data an EC10 of 0.037 mg/L was calculated, equipollent to the NOEC.
Executive summary:

In a flow through study, conducted based on US-EPA procedure 'Methods for acute  toxicity tests with fish, macroinvertebrates, and amphibians;  EPA-660/3-75-009 (1975), a 30 day LOEC of 0.055 mg/L was determined for the sac fry stage regarding the endpoints length and weight. From the test raw data an EC10 of 0.037 mg/L was calculated, equipollent to the NOEC.

Description of key information

The study started with 10 d old sac frys and lasted 30 days and is considered part of an Early Life Stage Test. No NOEC was obtained with the triphenyl phosphate concentrations tested. With the raw data given in the test protocol, an EC10 of 0.037 mg/L can be calculated, equipollent to NOEC according to Guidance on information requirements and chemical safety assessment by the European Chemicals Agency, 2008.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
0.037 mg/L

Additional information