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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study conducted according to national standard methods without detailed documentation (pH, dissolved oxygen and water hardness of test medium not stated) - (also assessed by OECD)
Qualifier:
according to
Guideline:
other: see below
Principles of method if other than guideline:
EPA 660/3-75-009: Method for acute toxicity tests with fish, macroinvertebrates and amphibians (1975)
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Named in reference as Salmo gairdneri the former name for Rainbow trout - no data given on size etc.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
No data given
Test temperature:
No data given
pH:
No data given
Dissolved oxygen:
No data given
Salinity:
Not aplicable
Nominal and measured concentrations:
No data given
Details on test conditions:
Test carried out according to "Committee on methods for acute toxicity tests with aquatic organisms" - Method for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians, Ecological Research Series, EPA-660/3-75-009, US EPA (1975)
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
95% confidence limit 96h LC50: 0.28-0.50 mg/L
95% confidence limit 96h LC50: 0.28-0.50 mg/L (0.4mg/L)
Conclusions:
The LC50 for rainbow trout has been determined as 0.4 mg/L
Executive summary:

The study was performed according to EPA 660/3-75-009: Method for acute toxicity tests with fish,  macroinvertebrates and amphibians (1975) to assess the acute toxicity of triphenyl phosphate to Oncorhynchus mykisss under static conditions. The LC 50 after 96 hours was calculated as 0.4 mg/L.

Description of key information

Mayer et al. (1981) reports static test results with 96 h-LC50 values of 0.4 mg/L for Oncorhynchus mykiss and 0.32 - 0.56 mg/L for the marine species Cyprinodon variegatus (both nominal concentrations).

Key value for chemical safety assessment

LC50 for freshwater fish:
0.4 mg/L
LC50 for marine water fish:
0.32 mg/L

Additional information

Several acute fish tests were performed according to the U.S. guideline EPA-660/3-75-009. Mayer et al. (1981) reports static test results with 96 h-LC50 values of 0.4 mg/L for Oncorhynchus mykissand 0.32 - 0.56 mg/L for the marine species Cyprinodon variegatus (both nominal concentrations). Further acute fish toxicity data were generated in studies on killifish (Oryzias latipes) and goldfish (Carassius auratus) (Sasaki et al, 1982), Oncorhynchus mykiss(Palawski et al, 1983) and Lepomis macrochirus (Huckins et al, 1991). These studies reported LC50 values that are broadly comparable with those reported in the key study by Mayer et al. (1981) presented here.