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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: study well documented, meets generally accepted scientific principles (also assessed by OECD)
Qualifier:
no guideline followed
Principles of method if other than guideline:
Continuous flow-through test
GLP compliance:
no
Specific details on test material used for the study:
Triphenyl phosphate purchased commercially
Radiolabelling:
no
Details on sampling:
Groups of 3 to 4 fish taken at intervals. The fish were analyzed after being stored in a freezer and water was analyzed immediately after sampling.
Details on preparation of test solutions, spiked fish food or sediment:
Acetone solutions were diluted with water to give a concentration of 1 ppm TPP. Tap water was used after dechlorination
Test organisms (species):
Oryzias latipes
Details on test organisms:
Oryzias latipes, Killifish, weighing 0.1 - 0.2g were acclimatised for at least 10 days
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
18 d
Total depuration duration:
1 d
Hardness:
No data given
Test temperature:
25 °C
pH:
No data given
Dissolved oxygen:
No data given
TOC:
No data given
Salinity:
Not applicable
Details on test conditions:
The test vessel consisted of a 10 L glass tank , TPP in acetone was mixed with water and was introduced into the test system at the rate of 1 - 2 L /h.
70 - 100 fish were used
Nominal and measured concentrations:
0.01 mg/L
Reference substance (positive control):
no
Details on estimation of bioconcentration:
Calculated from amounts of TPP in fish and water
Type:
BCF
Value:
144 dimensionless
Basis:
whole body w.w.
Remarks on result:
other: Concentration of TPP increased gradually over the 18 day period
Remarks:
Conc.in environment / dose:0.01 mg/L
Elimination:
yes
Depuration time (DT):
24 h
TPP uptake in fish was rapid and increased gradually till day 18 of  exposure 
BCF after 2 d: ca. 60; after 18 d: 144). The authors suggest  that this 
phenomenon is due to some alteration of metabolism or reduced  biotransformation 
by the fish with increase of exposure time. After the  fish were transferred to 
clean water, fast elimination occured and TPP concentrations in fish body 
decreased to levels below the detection limit within 24 h. The biological 
half-life is given to 1.2 h.
Conclusions:
TPP is gradually taken up over time giving a BCF of about 60 after 2 days and 144 after 18 days. When placed in clean water, levels of TPP in the fish fall rapidly and are below the detection limit within 24 hours.
Executive summary:

Uptake of TPP in Oryzias latipes, Killifish, was rapid and increased gradually over time giving a BCF of about 60 after 2 days and 144 after 18 days. After transferring the fish to clean water fast elimination occured and concentration of TPP is below the detection limit within 24 hours. The biological half-life is 1.2 h.

Description of key information

Uptake of TPP in Oryzias latipes, Killifish, was rapid and increased gradually over time giving a BCF of about 60 after 2 days and 144 after 18 days. After transferring the fish to clean water fast elimination occured and concentration of TPP is below the detection limit within 24 hours. The biological half-life is 1.2 h.

Key value for chemical safety assessment

BCF (aquatic species):
144 dimensionless

Additional information