Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
no glp, no guideline a available (older than guideline and glp) water from lakes was used for experiment; In 1972 water from lakes contained high amounts of chelating agent (e.h. EDTA) Chelating agent stabilze Sn(II) and avoid the formation of hydroxide. So the amount of Sn(II) in water in the study measured is higher than the solubility of Sn(II) at this pH.(see discussion in endpoint summary Environmental fate and pathways)
Justification for type of information:
Justification for read across: stannous sulphate and stannous chloride are the salts from strong acids (here: hydrochloric and sulfuric acid) with Sn(II). In water there is a complete dissociation in the Sn(II) and the corrospondenting acid.
In the study water phase the concentration of chloirde and sulfate from natural sources is dimension granter than from the test item. So the chloirde and the sulfate is insignificant. For adverse effects only the Sn(II) is responsible

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1972
Report Date:
1972

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
not applicable
Principles of method if other than guideline:
For chronic studies five daphnia 12 +/- 12 hr old were placed in 200 ml water in a 250 ml beaker. Four beakers (the test chambers) were used with total 20 animals for each experimental and control condition for each test. The test chambers were covered wirh a pane glase to minimize evaporation, and distributed randomly on a shelf in a constant temperature (18°C +/- 1) Young daphnia were placed in the test chambers, transferd into a new medium at the beginning of each week with a medicine dropper, an subjected to test conditions for 3 wks. Surival of the initial animals was noted after 1, 2 and 3 wks. At the end of the week 3 the surival animals was weighed and prepared for protein and GOT analyses. Reproduction was accessed by counting the young produced every week; the young was counted and discarded each time the inital animals were transfered and at the end of each experiment
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 wk

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 wk
Dose descriptor:
other: Chronik, 16% reproductive impairment
Effect conc.:
0.35 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
reproduction
Remarks on result:
other: value for Sn2+
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.18 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
dissolved
Remarks:
Sn
Basis for effect:
reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The value for 16% reproductive impairment was determinated to be 0.35 mg Sn^{+2} /L = 0.63 mg SnSO_4 / L

NOEC(21d)=0.18mg/L