Registration Dossier

Physical & Chemical properties

Additional physico-chemical information

Administrative data

Endpoint:
additional physico-chemical information
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
5.3.1996-28.3.1996
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Report Date:
1996

Materials and methods

Test guideline
Guideline:
other: density/refractive index/concentration - relationship measurement
Deviations:
no
Remarks:
14 observations; regression statistics were provided for the mathematical equation developed for the concentration-density relationship.
Principles of method if other than guideline:
The concentration -density relationship was determined by preparing 14 different water solutions of the substance. Solutions were prepared into the volumetric flasks (100 ml) stabilised at the water bath at temperature 20°C. The samples were weighted into the flasks, and filled up with water. The concentration range of the dilutions varied between 0 and 55 g/100 g. Dilutions were kept at the water bath (at 20°C) for 30 min. After that the flasks were weighed. Similar weighing was done also at temperatures 40°C and 60°C. In addition, the water content of the crystals were determined by Karl-Fisher titration. The water content (%) was calculated from the titration results. After that, the weight of the water solutions were corrected as a dry-weight of the substance in water solutions. This was done for each test solution by dividing the dry-weight with the weight of the solutions at temperatures 20, 40 and 60°C. This dry-weight-value was assumed to be constant during the hole measurement.

Expression of the results: The weight of the solutions (i.e. density) were obtained by the method described above. In addition, these densities were corrected with the deviation factor, i.e the density deviation from the known water density.

The mathematical equation was fitted for the density-concentration relationship using Quattro pro for Windows 5 - calculation program, and the regression statistics were calculated. The results are presented in section (any information including tables and figures).
GLP compliance:
no
Remarks:
company's own method
Endpoint investigated:
other: density of substance in water solutions

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Sample was pure. The stock number (company's ID) of the sample is 65115233 and the name of the test sample as referred in the report was crystallized farmabetaine, Naantali.

Results and discussion

Results:
The equation for the density-dry concentration relationship was determined by measuring the weight of the water solutions prepared at concentration range 0...55 g/100g of the substance. Concentrations in water solutions were correlated with the water content of the substance determined by Karl-Fisher-titration. The equations were developed for density-concentration (dry wieght) relationship at three temperatures 20, 40, and 60 C. The density of the water solution was 1.035 kg/dm-3 at concentration 20 g substance/100g . In addition, the density of the water solution was 1.092 kgdm-3 at concentration 50 g substance/100g.

Applicant's summary and conclusion

Conclusions:
The density-dry concentration relationship was determined by measuring the weight of the water solutions prepared at concentration range 0...55 g/100g of the substance. Concentrations in water solutions very corrected by the water content of the substance determined by Karl-Fisher-titration. The density-concentration (dry wieght) relationship was determined at three temperatures 20, 40, and 60 C. A mathematical equation was fitted for the density-concentration relationship results. The density results of two main concentrations, in which the substance is used, are the following: The density of the water solution was 1.035 kg/dm-3 at concentration 20 g substance/100g, and 1.092 kgdm-3 at concentration 50 g substance/100g.
Executive summary:

The density-dry concentration relationship was determined by measuring the weight of the water solutions prepared at concentration range 0...55 g/100g of the substance. Concentrations in water solutions very corrected by the water content of the substance determined by Karl-Fisher-titration. The density-concentration (dry wieght) relationship was determined at three temperatures 20, 40, and 60 C. A mathematical equation was fitted for the density-concentration relationship results. The density results of two main concentrations, in which the substance is used, are the following: The density of the water solution was 1.035 kg/dm-3 at concentration 20 g substance/100g, and 1.092 kgdm-3 at concentration 50 g substance/100g.