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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

No effect on long-term toxicity to aquatic invertebrates up to the limit of water solubility.

Key value for chemical safety assessment

Additional information

Several long-term freshwater studies on Daphnia magna according to OECD 202 are available for the category. No effects were observed up to the limit of water solubility and a NOEC(21d) > 1 mg/L could be stated.

The key study investigated the long-term effects of 2-ethylhexyl oleate (CAS 26399 -02 -0) to the freshwater invertebrate Daphnia magna (Kuech 1995). The study was performed according to the OECD guideline 202, part 2 of 1993. Test concentrations of nominal 1 and 100 mg/L were appplied as filtered test solutions. Analytically, concentrations of <0.01 mg/L were retrieved from both nominal concentrations.

Effects to Daphnia magna were observed after 21 days with no effect at 1 mg/l and 90% mortality (on day 19) at 100 mg/L. The authors stated, that the deaths in the higher concentration could be attributed to sample contamination. Therefore, a NOEC(21 d, mortality) > 1 mg/L (nominal) was reported. Similarly, 23% reduction of reproduction (number of offspring) in the 100 mg/L sample occurred after 21 days, with no effect in the 1 mg/L sample resulting in a NOEC(21 d, reproduction) > 1 mg/L. If the reproduction up to day 17 in the 100 mg/L sample was compared to the reproduction in the control, no reduction of reproduction was stated.