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Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

No acute toxicity effects up to the limit of water solubility.

Key value for chemical safety assessment

Additional information

There are no studies available on the short-term toxicity of 2 -ethylhexyl oleate (CAS 26399 -02 -0) to freshwater fish. The assessment was therefore based on studies conducted with the category members Fatty acids, C16-18 and C18-unsatd., 2-ethylhexyl esters (CAS 85049-37-2) and Fatty acids, C8-16, 2-ethylhexyl esters (CAS 135800-37-2), as part of a read across approach, which is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Grouping of substance and read across approach. Further justification is given within the endpoint summary 6.1 and within the category justification section 13. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was entered into IUCLID. Nevertheless, as can be seen in the data matrix of the category justification in Section 13, all reliable data in the category support the hazard assessment for this endpoint, by showing a consistent pattern of results.


In the selected read-across study with Fatty acids, C16-18 and C18-unsatd., 2-ethylhexyl esters (CAS 85049-37-2) the acute toxicity to Danio rerio was tested according to OECD 203 with nominal concentrations of 1000, 3000 and 10000 mg/L. The test substance was directly added into the test medium and tested as an emulsion. No mortality was observed up to the highest test concentration and an LC50(96h) > 10000 mg/L could be stated (Richterich 2001).

In another read-across from Fatty acids, C8-16, 2-ethylhexyl esters (CAS 135800-37-2), the marine species Scopthalmus maximus was tested with a WAF, resulting in a nominal LL50(96h) value > 5600 mg/L (Hudson 2002).

Additionally, a marine fish study is available for 2 -ethylhexyl oleate. The study was conducted according to OECD Guideline 203, using Scopthalmus maximus as test organism (Craig, 2002). No mortality occurred during the test, and the LC50 was determined to be > 1800 mg/L.

Thus, based on the above mentioned result, and due to the structural and profile similarities of the substances, as are explained with in the overall endpoint summary 6.1 it can be concluded that no toxicological effects on fish are expected up to the limit of water solubility for 2 -ethylhexyl oleate (CAS 26399 -02 -0).