N-(4-amino-9,10-dihydro-3-methoxy-9,10-dioxo-1-anthryl)benzenesulphonamide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Guideline: Mean of 2 determinations instead of 3

GLP compliance:

yes

Oxygen conditions:

other: not required as COD is being determined

Inoculum or test system:

other: not required as COD is being determined

Duration of test (contact time):

2 h

Details on study design:

A predeterminated amount of the test substance FAT - 36'034/D in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate.

The test substance was tested with a sample weight of 4.82 + 5.07 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/l.

Reference substance:

other: Potassium hydrogen phthalate solution (Merck-Nr. 11769)

Remarks on result:

not measured/tested

Parameter:

COD

Value:

1 747 mg O2/g test mat.

Validity criteria fulfilled:

not specified

Interpretation of results:

other: COD determined

Conclusions:

The COD of FAT 36034/D is found to be 1747 mg O2/g.

Executive summary:

The study describes the experimental techniques and the results obtained in a COD test of FAT 36034/D. The study was performed in accordance with DIN 38409 -H41 -1.

The COD of FAT 36034/D is found to be 1747 mg O2/g.

Description of key information

The COD of FAT 36034/D and reference substance was found to be 1747 mg O2/g and 196 mg O2/I respectively.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater

Additional information

A study was performed to determine the COD value of FAT 36034/D, by following the guideline DEV DIN 38409 - H 41-1 (1980) corresponding to 92/96/EE C C.6., in compliance with the Good Laboratory Practice (GLP) Regulations. A predetermined amount of the test substance FAT 36034/D in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate was determined by titration with standardized ferrous ammonium sulphate. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/l. The COD of FAT 36034/D and reference substance was found to be 1747 mg O2/g and 196 mg O2/I respectively. The study reported the value of greater than 1000 mg /g O2 for COD. Relevant is the BOD5/COD quotient which is not > 0.5 , therefore the substance is not indicative of rapid degradation. Taking all data into consideration, the test substance FAT 36034 is considered to be not biodegradable.