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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 - 16 Sep 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: EG Guideline 92/69 C.1
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1984
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: all tested concentrations at 0 and 24 h, concentrations 4.3, 6.0 and 8.5 mg/L at 48 h, concentrations 4.3, and 6.0 mg/L at 72 and 96 h
- Sampling method: samples was taken from the outflow of the mixing tank
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding 14 g test substance to 1 L purified water. The stock solutions were prepared daily and diluted with purified water to achieve the required test concentrations.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3 cm +/- 0.5 cm
- Weight at study initiation (mean and range, SD): approximately 0.25 g
- Feeding during test
- Food type: no feeding


ACCLIMATION
- Acclimation period: 14 days
- Type and amount of food: TetraMin at a concentration of 1% of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): <= 5% seven days prior test until test start

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
11 ° dH
Test temperature:
19 °C - 20 °C
pH:
8.0 - 8.2
Dissolved oxygen:
80% - 99%
Nominal and measured concentrations:
nominal: 4.3, 6.0, 8.5, 11.8 and 17.1 mg/L
mean measured (arith.): 4.6, 6.8, 13.9, 12.1 and 17.2 mg/L based on a.i.
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquaria
- Fill volume: approximately 45 L aquaria
- Aeration: continuous
- Renewal rate of test solution (flow rate): approximately 10 L/h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water - Gelsenwasser AG
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality after 24, 48, 72, and 96 h
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
7.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Details on results:
- Mortality of control: no
Sublethal observations / clinical signs:

Table 1: Mortality

Concentration [mg/L] Number of fish (initial) % mortality 
24 h 48 h 72 h 96 h
control 10 0 0 0 0
4.3 10 0 0 0 0
6 10 0 0 0 0
8.5 10 0 60 100 100
11.8 10 100 100 100 100
17.1 10 100 100 100 100

Table 2: Results analytical dose verification

Nominal concentration [mg/L] Measured concentration [mg/L]  
0 h 24 h 48 h 72 h 96 h mean 
4.3 5.4 3.6 6.5 3.9 3.4 4.6
6 7.7 4.4 10.8 6 5.1 6.8
8.5 10.8 6.8 24.2  --   --  13.9
11.8 13 11.1  --   --   --  12.1
17.1 22 12.3  --   --   --  17.2

-- : not determined, all fish dead

Table 3: Validity criteria for OECD 203.

Criterion from the guideline

Outcome

Validity criterion fulfilled

The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test

0%

yes

The dissolved oxygen concentration must have been at least 60 per cent of the air

saturation value throughout the test

80% - 99%

yes

There must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80% of the

nominal concentration throughout the test. If the deviation from the nominal

concentration is greater than 20%, results should be based on the measured

concentration.

> 80% based on MBAS

yes

 

Validity criteria fulfilled:
yes
Remarks:
The validity crietria according to OECD 203 are fulfilled (for details see table 3 under "any information on results")

Description of key information

In the key-study a LC50-value for fish of 7.1 mg a.s./L could be determined after 96 h after exposure. 

Key value for chemical safety assessment

Additional information

One reliable study on the toxicity of AES (C12-14, 1-2.5EO) Na to fish is available. The test was conducted with tap water according to EG Guideline 92/69 C.1 and OECD 203. Zebra fish (Danio rerio) were exposed to nominal substance concentrations of 4.3, 6.0, 8.5, 11.8 and 17.1 mg/L in a flow-trough system. The test concentrations were analytically verified using the MBAS assay (methylene blue active substances assay) as analytic test method. The mean measured concentrations were 4.6, 6.8, 13.9, 12.1 and 17.2 mg/L based on the active substance. The determined LC50 (96 h) was 7.1 mg a.s./L based on the nominal concentration.

In a second, supporting study which could not be fully evaluated as only a short summary is available, zebrafish were exposed in a static system (test following different guidelines). The resulting LC50 -value was in a range of 1 to 10 mg/L.

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