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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not applicable
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This study was conducted prior to GLP and test guidelines, but sufficient data are available for interpretation of results.
Justification for data waiving:
other:
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1948
Report date:
1948

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Test material was applied to the abdomen of rabbits and the animals were observed following exposure.
GLP compliance:
no
Remarks:
Conducted prior to GLPs
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-iminodi(ethylamine)
EC Number:
203-865-4
EC Name:
2,2'-iminodi(ethylamine)
Cas Number:
111-40-0
Molecular formula:
C4H13N3
IUPAC Name:
N-(2-aminoethyl)ethane-1,2-diamine
Details on test material:
Identification number: 550-A-2952.

Test animals

Species:
rabbit
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
Not data

Administration / exposure

Type of coverage:
not specified
Vehicle:
other: undchanged and an aqueous solution
Details on dermal exposure:
Clipped skin of the rabbit trunk
Duration of exposure:
Not indicated
Doses:
Undiluted and 10% aqueous dilution
No. of animals per sex per dose:
Six
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: no data
- Frequency of observations and weighing:no data
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs, body weight
Statistics:
Calculated by the method of Thompson.

Results and discussion

Preliminary study:
Not applicable
Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
1.09 mL/kg bw
Remarks on result:
other: range (0.95 to 1.24)
Mortality:
With a 10% dilution, one of 6 rabbits died an unexplained death at 1.0 gm/kg.
Clinical signs:
Immediate erythema, followed by necrosis and finally a leathery scab resulted from the direct action of the material on the skin. The stools of survivors were watery and sometimes blood-tinged.

With a 10% aqueous dilution the corrosive action on the skin was eliminated and none of 6 rabbits succumbed to a dosage of 2.0 gm/kg (20 ml/kg or the 10% dilution). One of 6 rabbits' died an unexplained death at 1.0 gm./kg. All lost weight save one, which gained only 4- grams. This demonstrates that the toxicity by skin absorption is enhanced by the corrosive action of the undiluted compound as the LD50 for a 10% dilution would be above 4 gm/kg.
Body weight:
With a 10% dilution, all lost weight save one, which gained only 4 grams.
Gross pathology:
In animals that died, the lungs were often congested and sometimes hemorrhagic; the kidneys showed considerable gross damage including a pitted appearance of the surface and subcapsular hemorrhage.
Other findings:
Not applicable

Any other information on results incl. tables

The application of undiluted diethylene tramine to the clipped skin of the rabbit trunk resulted in an LD50 of 1.09 (0.95 to 1.24) ml/kg calculated by the method of Thompson. Immediate erythema, followed by necrosis and finally a leathery scab resulted from the direct action of the material on the skin. In animals that died, the lungs were often congested and sometimes hemorrhagic; the kidneys showed considerable gross damage including a pitted appearance of the surface and subcapsular hemorrhage. The stools of survivors were watery and sometimes blood-tinged. With a 10% aqueous dilution the corrosive action on the skin was eliminated and none of 6 rabbits succumbed to a dosage of 2.0 gm/kg (20 ml/kg or the 10% dilution). One of 6 rabbits' died an unexplained death at 1.0 gm/kg. All lost weight save one, which gained only 4- grams. This demonstrates that the toxicity by skin absorption is enhanced by the corrosive action of the undiluted compound as the LD50 for a 10% dilution would be above 4 gm/kg.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Remarks:
Migrated information
Conclusions:
The LD50 for the undiluted compound in the rabbit-skin penetration test is 1.09 (0.95 to 1.24) ml/kg. Severe skin damage resulted from the undiluted compound. As a 10% dilution in water the corrosive action is practically eliminated and 2.0 gm/kg (20. ml/kg of the 10% dilution) is not lethal, demonstrating that skin damage abets skin penetration.
Executive summary:

The acute dermal toxicity of DETA was examined. The LD50 for the undiluted compound in the rabbit-skin penetration test is 1.09 (0.95 to 1.24) ml/kg. Using the density value of 958.6 kg/m3(IUCLID section 4.4) this corresponds to 1045 mg/kg (range 910 mg/kg to 1190 mg/kg). Severe skin damage resulted from the undiluted compound.

As a 10% dilution in water the corrosive action is practically eliminated and 2.0 gm/kg (20 ml/kg of the 10% dilution) is not lethal, demonstrating that skin damage abets skin penetration.