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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August to December 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Samples for analysis of new and old test solutions were taken new at 0 hours (test initiation) and old at 48 hours (test termination).
Vehicle:
no
Details on test solutions:
The water accommodated fractions of the test material were prepared by stirring the test material at each loading level in the exposure solution for 44 hours with a 1.5 to 2 hour settling period, after which each aqueous phase was removed and added to a test system. A second 10 mg/L WAF was stirred for 25 hours with a 1.5 to 2 hour settling period because the original WAF vessel developed a leak as it was stirring. An equilibration study was conducted over a period of 72 hours prior to the definitive study to determine the approximate length of time needed to stir the test substance in the test media to acheive a saturated solution. Results suggested that a stirring period of 20 to 28 hours would be sufficient to reach equilibrium.
Test organisms (species):
Daphnia magna
Details on test organisms:
Organisms used in the test were from a laboratory culture were less than 24 hour old. The source of the culture was from Zeneca Brixham Laboratory from I.R.Ch.A., France.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
155 mg/L CaCO3
Test temperature:
19.3 to 19.7 degrees C
pH:
7.1 to 8.2
Dissolved oxygen:
9.1 to 9.2 mg/L
Nominal and measured concentrations:
Nominal loading rates were 1.0, 2.2, 4.6, 10, 22, 46, and 100 mg/L. A control was also included. Based on analyses of new and old test solution samples taken new at 0 hours (test initiation) and old at 48 hours (test termination), the nominal/measured (mean) concentration values were: control/<0.02 mg/L; 1.0/0.11 mg/L; 2.2/0.22 mg/L; 4.6/0.18 mg/L; 10/0.25 mg/L; 22/0.44 mg/L; 46/0.47 mg/L; and 100/0.56 mg/L.
Details on test conditions:
The study was conducted in closed test systems that were not renewed during the study. The test systems used were 150 ml glass Erlenmeyer flasks. The control and treatment level was evaluated in replicate test systems. Ten organisms, less than 24 hours old, were exposed in each of the control and treatment solution flasks and were not fed during the study.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
LL0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 22 - < 46 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EL0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
A total of 20 daphnids were added to the control and treatment solutions. Immobility results are as follows:

Loading Rate % Immobility
(mg/L) 24 hr 48 hr
Control 0 0
1.0 0 0
2.2 0 0
4.6 0 0
10 0 0
22 5 25
46 10 65
100 25 75
Reported statistics and error estimates:
Effect levels were estimated by visual inspection of the data, which is thought to be more appropriate than interpolative statistical analysis if the data are based on water accommodated fractions of a complex test substance with low water solubility.
Validity criteria fulfilled:
yes
Conclusions:
Water accommodated fractions (WAFs) of the test substance produced a 48-hour EL50 range of >22 to <46 mg/L and a LL0 value of 10 mg/L with Daphnia magna. There was no mortality in the control.
Executive summary:

Water accommodated fractions (WAFs) of the test substance produced a 48-hour EL50 range of >22 to <46 mg/L and a LL0 value of 10 mg/L with Daphnia magna. There was no mortality in the control.

Description of key information

The water accommodated fraction (WAF) of hydrocarbons, C9-C10, n-alkanes, isoalkanes, cyclics, <2% aromatics, produced a 50% effect (immobility) with Daphnia magna at a loading of >22-<46 mg/L after a 48-hour exposure.

Key value for chemical safety assessment

Additional information

The water accommodated fraction (WAF) of hydrocarbons, C9-C10, n-alkanes, isoalkanes, cyclics, <2% aromatics, produced a 50% effect (immobility) with Daphnia magna at a loading of >22 -<46 mg/L after a 48-hour exposure.