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Data on the acute aquatic toxicity of ethylene oxide are available for fish and Daphnia. Since there is no study concerning the toxicity of ethylene oxide in algae a read-across to propylene oxide (CAS 75 -56 -9) was applied (for a read-across justification see attached justification document in IUCLID chapter 13). The data demonstrate that fish are the most sensitive organisms with a LC50 (96 h) value of 84 mg/L (Conway et al., 1983). Daphnia was less sensitive with an EC50 (48 h) value of 212 mg/L (Conway et al., 1983) and the EC50 for the growth rate of algae exposed to propylene oxide for 96 hours was 240 mg/L (Shell Research Ltd. 1986).