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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Japanese government peer-reviewed the documents, audited selected studies.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Supplier: KISHIDA CHEMICALS CO., Ltd (Lot No. D12358J)
Purity >= 98 %
Analytical monitoring:
yes
Details on sampling:
-concentration: 0, 1000 mg/L
-renewal of test solution: none
Details on test solutions:
STOCK AND TEST SOLUTION AND THEIR PREPARATION:
-solvent: no solvent was used
-stock solution: no data
DILUTION WATER:
-source: M4 medium (refer to OECD-TG 211)
Test organisms (species):
Daphnia magna
Details on test organisms:
Test Organism:
-supplier: National Institute of Environmental Studies (Japan)
-age: juvenile Daphnia magna less than 24 hr old
-feeding during acclimation: Chlorella vulgaris, 0.1-0.2 mgC/day/individual
-acclimation: 21 days
-feeding during test: none
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
249 mg/L as CaCO3
Test temperature:
19.9 - 20.6 °C
pH:
7.4 - 7.5
Dissolved oxygen:
8.4 - 8.9 mg/L
Salinity:
no data
Nominal and measured concentrations:

Results:
Table NOMINAL/MEASURED CONCENTRATION
-----------------------------------------------------------
Nominal conc. Measured conc. (mg/L)
(mg/L) (% of nominal)
0 hr* 48 hr**       mean
-----------------------------------------------------------
Control <0.5 <0.5
1000 mg/L 984.7 (98.5) 892.6 (89.3) 937.5
-----------------------------------------------------------
* : freshly prepared test solution
**: test solution after 48hr exposure period
Details on test conditions:
-concentration: 0, 1000 mg/L
-renewal of test solution: none
-exposure vessel type: 100 mL test solution in a 100 mL glass vessel
-number of replicates/individual per replicate: 4/5
-intensity of irradiation: room light -photoperiod: 16hr - 8hr light-dark cycle
TEST PARAMETER: immobility
SAMPLING: at start and end of test
Reference substance (positive control):
yes
Remarks:
K2Cr2O7
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:

EFFECT DATA (IMMOBILIZATION)
24hr EiC50 > 1000 mg/L
48hr EiC50 > 1000 mg/L
48hr NOEiC > 1000 mg/L

MORTALITY OR IMMOBILITY
No mortality and immobility at Control and 1000 mg/L of 2-Methyl-2-propenamide
Results with reference substance (positive control):
EC50(48h)=0.60 mg/l
Reported statistics and error estimates:
no data
Validity criteria fulfilled:
yes
Conclusions:
At a limit concentration of 1000 mg/l (nominal), methacrylamide showed no mortality in a static Daphnia sp. acute immobilisation test OECD 202
EC50 (48h) > 1000 mg/l (measured/nominal)
Executive summary:

The 48–hr-acute toxicity of methacrylamide to Daphnia magna was studied under static conditions. Daphnids were exposed to control and test item at nominal/measured concentration of 1000 mg/l for 48 hr.  Immobilization was observed daily.  The 48–hour EC50 was > 1000 mg a.i./L.  The 48– hr NOEC based on immobilization effects was 1000 mg a.i./L.   

Based on the results of this study, methacrylamide would  be classified as not acute toxic to invertebrates in accordance with the classification system of the CLP regulation. 

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

Results Synopsis

Test Organism Age: Daphnia magna less than 24 hr old

Test type: Static

EC50 (24hr): > 1000 mg/l

EC50 (48hr): > 1000 mg/l

NOEC (48 hr) = 1000 mg/l

Description of key information

The 48–hr-acute toxicity of methacrylamide to Daphnia magna was studied under static conditions. Daphnids were exposed to control and test item at nominal/measured concentration of 1000 mg/l for 48 hr.  Immobilization was observed daily.  The 48–hour EC50was > 1000 mg a.i./L.  The 48– hr NOEC based on immobilization effects was 1000 mg a.i./L.   

EC50 (48hr): > 1000 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1 000 mg/L

Additional information

EC50 (24hr): > 1000 mg/l

NOEC (48 hr) = 1000 mg/l