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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

LC50 fish (96h): > 100 mg/l

EC50 daphnia (48h): > 1000 mg/l

NOEC daphnia (21d): > 100 mg/l

ErC50 algae (72h): > 1000 mg/l

NOErC algae (72h): 1000 mg/l

EC50 microorganisms (3h): 995 mg/l

Additional information

Acute aquatic tests are available for all three trophic levels. Chronic data are available with Daphnia magna and algae.

Methacrylamide is not toxic to aquatic organisms and plants.


In a 96-h acute toxicity study, Medaka (Oryzias latipes) were exposed to methacrylamide at nominal concentrations of > 100 mg/l without solvent under semistatic conditions. The 96-h LC50 was > 100 mg/l.

Based on the results on this study, methacrylamide would be classified as not acute toxic to fish in accordance with th classification CLP regulation.

This toxicity study is classified as acceptable and satifies the guideline for Fish, acute toxicity study (OECD 203)

LC50: > 100 mg/l measured/nominal


The 48–hr-acute toxicity of methacrylamide to Daphnia magna was studied under static conditions. Daphnids were exposed to control and test item at nominal/measured concentration of 1000 mg/l for 48 hr.  Immobilization was observed daily.  The 48–hour EC50was > 1000 mg a.i./L.  The 48– hr NOEC based on immobilization effects was 1000 mg a.i./L.   

EC50 (48hr): > 1000 mg/l


In a 72-hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata, strain ATCC-22662 were exposed to control and test item at nominal/measured concentrations of 1000 mg a.i./L in accordance with the Algae, Growth Inhibition Test, OECD 201  The NOEC and ErC50values based on growth inhibition were 1000 mg/l and > 1000 mg a.i./L, respectively.  The % growth inhibition in the treated algal culture as compared to the control ranged from -2.08 to 1.59 

There were no compound related phytotoxic effects.


The 21-day-chronic toxicity of methacrylamide to Daphnia magna was studied under static renewal conditions. Daphnids were exposed to control and test chemical at nominal/measured concentrations of 100 mg a.i./L.  The 21 day LC50/EC50based on mortality/sublethal effect was > 100 mg a.i./L.  The 21-day NOEC based on sublethal effect was >100 mg a.i./L.  The sublethal effects included were reproduction.  Production of offsprings in the treated groups indicated that methacrylamide did not have an effect on the reproduction at concentrations greater than > 100 mg a.i./L. 


In a vaild respiration inhibition test with activated sludge (OECD 209) Methacrylamide was tested at concentrations of31.3, 62.5, 125, 250, 500, 1000 and 2000 mg/l (nominal concentrations). EC50 (3h) was 995 mg/l, EC10 (3h) was 37 mg/l.