1,3-Isobenzofurandione, hexahydro-, reaction products with epichlorohydrin

Biodegradation in water: screening tests

Description of key information

Ready Biodegradability of bis(2,3-epoxypropyl)cyclohexane-1,2-dicarboxylate in a Manometric Respirometry Test

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Additional information

In first study, the substance was tested for its ready biodegradability in the CO2 Evolution Test (Modified Sturm Test) at ca. 42 mg / 2L, corresponding to approx 12 mg TOC / L. The relative degradation values calculated from the measurement of the CO2 formation during the test period of 28 days revealed no significant degradation (4.9% in bottle A and 2.6% in bottle B). The substance was found not readily biodegradable.The toxicity test showed that there is no inhibition of the degradation of sodium acetate by the test substance. Therefore, bis(2,3 -epoxypropyl)cyclohexane-1,2 -can be considered to be inherently biodegradable.

In a second study, the ready biodegradability was tested in a Manometric Respirometry Test. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the 10-day window, describing the period between reaching at least 10% degradation and 60% degradation. This period should not exceed 10 days.

The mean biodegradation after 28 days of bis(2,3 -epoxypropyl)cyclohexane-1,2 -dicarboxylate was 53% (ThOD_NH4) and 94% after 60 days; the 10 day window criterion was not passed.

Whilst bis(2,3 -epoxypropyl)cyclohexane-1,2 -dicarboxylate cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F, the evidence of aerobic degradation (94%) that did not plateau before the end of the 60-day indicate the potential for complete mineralisation in the environment.