Butyl acrylate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given: scientifically acceptable study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Principles of method if other than guideline:

BASF test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Butyl acrylate

EC Number:

205-480-7

EC Name:

Butyl acrylate

Cas Number:

141-32-2

Molecular formula:

C7H12O2

IUPAC Name:

butyl prop-2-enoate

Test material form:

other: liquid

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler, 6050 Offenbach, Germany
- Weight at study initiation: approx. 3.1 kg (mean)
- Sex: male / female
- Diet (ad libitum): Ssniff-K-standard diet/rabbit Intermast, Soest
- Water (ad libitum): tap water

Test system

Type of coverage:

occlusive

Preparation of test site:

other: clipped

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin sites of the same animals

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): undiluted, no amount specified

Duration of treatment / exposure:

1 min, 5 min, 15 min, and 20 h

Observation period:

8 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: 2.5 x 2.5 cm
- Type of wrap if used: patch, no further data


REMOVAL OF TEST SUBSTANCE
- Washing: yes (exposure 1, 5, 15 min) first with undiluted Lutrol 9, then with an aqueous Lutrol solution (50 %); no (exposure 20 h)
- Time after start of exposure: 1, 5, and 15 min, respectively


SCORING SYSTEM:
The original BASF grading was converted into the numerical grading according to the OECD Draize system.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Remarks:

15 min exposure

Basis:

mean

Remarks:

(2 animals)

Time point:

other: 24-48-72 h

Score:

3.9

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Results 2

Irritation parameter:

edema score

Remarks:

15 min exposure

Basis:

mean

Remarks:

(2 animals)

Time point:

other: 24-48-72 h

Score:

2.9

Max. score:

4

Reversibility:

fully reversible within: 8 days

Results 3

Irritation parameter:

erythema score

Remarks:

20 h exposure

Basis:

mean

Remarks:

(2 animals)

Time point:

other: 24-48-72 h

Score:

3.9

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: 1st Trial

Results 4

Irritation parameter:

edema score

Remarks:

20 h exposure

Basis:

mean

Remarks:

(2 animals)

Time point:

other: 24-48-72 h

Score:

3

Max. score:

4

Reversibility:

fully reversible within: 8 days

Remarks on result:

other: 1st Trial

Irritant / corrosive response data:

Twenty four hours following the 1-, 5- and 15-minute applications strong erythema and edema were seen. The 20-hour exposure (trial 1) caused slight necrosis in addition to erythema and edema. Six and eight days after the applications, slight scaling was observed for the 1-, 5- and 15 -minute applications. The 20-hour application (trial 1) caused parchment like necrosis of the upper skin layer and scaling. The trial 2 of 20 h application resulted into similar responses as observed in trial 1.

Any other information on results incl. tables

                                            Skin Irritation Score, Rabbit 1/2, Erythema

Exposure time	24 h	48 h	72 h	Mean
1 min	4a/3a	4a/3a	1/0	3/2
5 min	4a/4a	4a/4a	2a/4	3.3/4
15 min	4a/4a	4/4a	3a/4	3.7/4
20 h (trial 1)	4a/3a	4b/4b	4b/4b	4/3.7

Skin Irritation Score, Rabbit 1/2, Edema

Exposure time	24 h	48 h	72 h	Mean
1 min	3a/3a	3a/3a	0/3	2/3
5 min	3a/3a	3/3a	2a/2	2.7/2.7
15 min	3a/3a	3/3a	3a/2	3/2.7
20 h (trial 1)	3/3	3/3	3/3	3/3

a- extending beyond the area of application

b- livid