1,4-diisopropylbenzene

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Remarks:

in vivo study that was performed prior to current requirement for in vitro alternative testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 January 1982 - 16 April 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 January 1982 - 16 April 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

standard acute method

Specific details on test material used for the study:

TEST MATERIAL
- name as cited in test report: p-diisopropylbenzene
- Molecular weight: 162 g/mol
- Molecular formula: C12H18
- Physical state: liquid
- Boiling point: 208°C at 760 mmHg

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability with H+; OH-; Heat, Light, H2: given

Species:

rat

Strain:

not specified

Sex:

male/female

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Remarks:

100 % as received

Doses:

3200 mg/kg

Control animals:

no

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 3 200 mg/kg bw

Based on:

test mat.

Mortality:

No mortalities were observed.

Clinical signs:

other:

Gross pathology:

No necropsies were conducted

The acute oral LD50 of this compound was greater than 3200 mg/kg in male and female rats. Clinical signs of toxicity included slight to moderat weakness and rough coats.

Interpretation of results:

other: EU GHS criteria not met

Conclusions:

Based on the results of this study, the acute oral LD50 for test item was greater than 3200 mg/kg in male and female rats. Clinical signs of toxicity included slight to moderate weakness and rough coats. 

Executive summary:

An acute oral toxicity study was conducted on rats. Based on the results of this study, the acute oral LD50 for test item was greater than 3200 mg/kg in male and female rats. Clinical signs of toxicity included slight to moderate weakness and rough coats. 

Cross-reference 2

Reason / purpose for cross-reference:

reference to same study

Reference

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 January 1982 - 16 April 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

no guideline followed

Principles of method if other than guideline:

The test was conducted according to an internal method.

GLP compliance:

not specified

Specific details on test material used for the study:

TEST MATERIAL
- name as cited in test report: p-diisopropylbenzene
- Molecular weight: 162 g/mol
- Molecular formula: C12H18
- Physical state: liquid
- Boiling point: 208 °C at 760 mmHg

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability with H+; OH-; Heat, Light, H2: given

Species:

guinea pig

Strain:

not specified

Sex:

not specified

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

occluded, single exposure, 24 hours
(guinea pig abdomen)

Duration of exposure:

24 hours

Doses:

single dose (dose up to 20 mL/kg)

Control animals:

no

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 20 mL/kg bw

Based on:

test mat.

Remarks on result:

other: = ca. 20000 mg/kg bw/d

Clinical signs:

other: erythema, edema, necrosis, eschars, scarring, desquamation and aloepcia

It produced moderate to severe erythema and edema and some necrosis initially; eschars were present after one week, and scarring, desquamation and alopecia were present after two weeks at doses ranging from 20 mL/g down to 1 mL/kg.

The dermal LD50 was greater than 20 mL/kg. 

Interpretation of results:

GHS criteria not met

Conclusions:

Based upon the results of this test, the acute dermal LD50 of test item was determined to be greater than 20 mL/kg bw (= ca. 20000 mg/kg bw/d).

Executive summary:

The acute toxicity to guinea pig in dermal test was performed. At most, test item is only slightly toxic by the dermal route. There was no evidence of percutaneous absorption. LD50 value was determined to be greater than 20mL/kg bw (= ca. 20000 mg/kg bw/d).

Cross-reference 3

Reason / purpose for cross-reference:

reference to same study

Reference

Endpoint:

skin irritation: in vivo

Remarks:

in vivo study that was performed prior to current requirement for in vitro alternative testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 January 1982 - 16 April 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

no guideline followed

Principles of method if other than guideline:

The test was conducted according to an internal method.

GLP compliance:

no

Specific details on test material used for the study:

TEST MATERIAL
- name as cited in test report: p-diisopropylbenzene
- Molecular weight: 162 g/mol
- Molecular formula: C12H18
- Physical state: liquid
- Boiling point: 208 °C at 760 mmHg

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability with H+; OH-; Heat, Light, H2: given

Species:

guinea pig

Strain:

not specified

Type of coverage:

occlusive

Preparation of test site:

other: depilated

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

single dose

Duration of treatment / exposure:

24 hours

Observation period:

2 weeks

Details on study design:

occluded, single exposure, 24 hours
(guinea pig abdomen)

and

open, Repeated (10 doses) - 5 animals

Irritation parameter:

overall irritation score

Basis:

animal:

Remarks on result:

other: Moderate erythema, edema and some necrosis.

Irritation parameter:

edema score

Basis:

animal:

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Irritation parameter:

erythema score

Basis:

animal:

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Irritant / corrosive response data:

The compound was a moderate skin irritant when applied to the depilated guinea pig abdomen under an occlusive wrap for 24 hours. It produced moderate erythema, edema and some necrosis. Thin eschars at one week, light scarring desquamation and alopecia at 2 weeks.

Occluded, single dose:

The compound produced moderate to severe erythema and edema and some necrosis initially; eschars were present after one week, and scarring, desquamation and alopecia were present after two weeks at doses ranging from 20 mL/g down to 1 mL/kg.

 

Open, Reapead (10 Doses) - 5 animals:

Moderate to severe erythema and edema replaced by thick, broken eschars at day 10.

Skin Absorption possible

 

Repeated daily application (10) of 0.5 mL of the material moderately exacerbated the initial response. Moderate to severe erythema and edema and petechial hemorrhages were present at day 1. By day 10, the application site in all animals was characterized by thick, broken eschars. Absorption of the compound through the skin was possible, since three of five animals in the repeated skin application test lost weight over the course of the study. 

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

p-diisopropylbenzene was rated a moderate irritant under the conditions of this study.

Executive summary:

p-diisopropylbenzene was a moderate skin irritant when applied to the depilated guinea pig abdomen under an occlusive wrap for 24 hours.

It produced moderate erythema, edema and some necrosis. Thin eschars at one week, light scarring desquamation and alopecia at 2 weeks.

Cross-reference 4

Reason / purpose for cross-reference:

reference to same study

Reference

Endpoint:

skin sensitisation: in vivo (non-LLNA)

Remarks:

in vivo study that was performed prior to current requirement for in vitro alternative testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 January 1982 - 16 April 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 406 (Skin Sensitisation)

Deviations:

yes

Remarks:

only 10 animals tested, not many details provided in the report

Principles of method if other than guideline:

only 10 animals tested, not many details provided in the report

GLP compliance:

not specified

Type of study:

guinea pig maximisation test

Justification for non-LLNA method:

in vivo study that was performed prior to current requirement for in vitro alternative testing

Specific details on test material used for the study:

TEST MATERIAL
- name as cited in test report: p-diisopropylbenzene
- Molecular weight: 162 g/mol
- Molecular formula: C12H18
- Physical state: liquid
- Boiling point: 208 °C at 760 mmHg

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability with H+; OH-; Heat, Light, H2: given

Species:

guinea pig

Strain:

not specified

Sex:

not specified

Route:

epicutaneous, occlusive

Vehicle:

unchanged (no vehicle)

Concentration / amount:

100 % as received

Route:

epicutaneous, occlusive

Vehicle:

unchanged (no vehicle)

Concentration / amount:

100 % as received

Day(s)/duration:

not specified

No. of animals per dose:

10

Details on study design:

no details given

Positive control substance(s):

no

Reading:

other: not specified

Group:

positive control

Dose level:

not specified

Remarks on result:

other: not specified

Reading:

other: not specified

Group:

negative control

Dose level:

not specified

No. with + reactions:

0

Remarks on result:

other: not specified

Reading:

other: not specified

Group:

test chemical

Dose level:

not specified

No. with + reactions:

0

Total no. in group:

10

Remarks on result:

other: not specified

The material did not elicit a positive response when administreed to ten guinea pigs in a standardized skin sensitization test. 

Interpretation of results:

GHS criteria not met

Conclusions:

Based upon the results of this study, test item is not a dermal sensitizer in guinea pigs

Executive summary:

A skin sensitisation test was performed on guinea pigs. The material did no elicit a positive response when administered to ten guinea pigs in a standardized skin sensitization test.

Consequently, estimated human risk is low and the test substance was determined to be non-sensitizing in guinea pigs.

Cross-reference 5

Reason / purpose for cross-reference:

reference to same study

Reference

Endpoint:

short-term repeated dose toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

26 January 1982 - 16 April 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 407 (Repeated Dose 28-Day Oral Toxicity Study in Rodents)

GLP compliance:

no

Limit test:

no

Specific details on test material used for the study:

TEST MATERIAL
- name as cited in test report: p-diisopropylbenzene
- Molecular weight: 162 g/mol
- Molecular formula: C12H18
- Physical state: liquid
- Boiling point: 198 °C at 760 mmHg

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability with H+; OH-; Heat, Light, H2: given

Species:

rat

Strain:

not specified

Sex:

male

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

Rats were given a total of 11 treatments over a 15-day span

Frequency of treatment:

once daily

Dose / conc.:

100 mg/kg bw/day (nominal)

Dose / conc.:

1 000 mg/kg bw/day (nominal)

No. of animals per sex per dose:

Groups of five male rats were gavaged with 1000 or 100 mg compound/kg/day for a total of eleven treatments over a 15 day span.
A control group of five animals were gavaged with distilled water at a dose of 1000 mg/kg/day. Weight gain and feed intake were comparable between groups.

Control animals:

yes, concurrent vehicle

Clinical signs:

effects observed, non-treatment-related

Description (incidence and severity):

No abnormal clinical signs were observed with the exception of a single high-dose animal exhibited porphyrin nasal discharge, weight loss and possible aspiration pneumonia.

Mortality:

no mortality observed

Body weight and weight changes:

no effects observed

Description (incidence and severity):

Weight gain and feed intake were comparable between groups.

Food consumption and compound intake (if feeding study):

no effects observed

Food efficiency:

not examined

Water consumption and compound intake (if drinking water study):

not examined

Ophthalmological findings:

not examined

Haematological findings:

effects observed, treatment-related

Description (incidence and severity):

several slight but statistically-significant differences were noted in the high-dose group, including increased platelets and monocytes. All other indices (red blood cell count, hemoglobin concentration, hematocrit, red cell indices, white blood cell counts and the remaining portions of the differential white blood cell count) were comparable to controls.
No alterations in hematology were noted in blood from the low dose group.

Clinical biochemistry findings:

effects observed, treatment-related

Description (incidence and severity):

A moderate decrease in serum glucose and a slight increase in creatinine in the high-dose animals; all other serum chemistry indices (alanine aminotransferase, aspartate aminotransferase, sorbitol dehydrogenase, alkaline phosphatase, and urea nitrogen) were comparable to controls.
No alterations in serum clinical chemistry were noted in blood from the low dose group.

Endocrine findings:

not examined

Urinalysis findings:

not examined

Behaviour (functional findings):

not examined

Immunological findings:

not examined

Organ weight findings including organ / body weight ratios:

effects observed, treatment-related

Description (incidence and severity):

No significant changes in absolute or relative kidney or spleen weights were noted.
There was a trend which implied a dose dependent increase in relative liver weights were observed in the high-dose group.

Gross pathological findings:

effects observed, non-treatment-related

Description (incidence and severity):

Enlarged hearts were observed in 3 of 5 rats in the high-dose group, which was considered to have occurred as a result of general respiratory stress associated with extensive consolidation of the lung (hypertrophy or the hearts might have been triggered by the consolidation in the lung).

Histopathological findings: non-neoplastic:

no effects observed

Description (incidence and severity):

Even though the enlarged hearts had been observed in the gross pathological examination, no compound related effects were seen at histopathological examination.

Histopathological findings: neoplastic:

not specified

Other effects:

not examined

Details on results:

The liver may be a site of toxic action, and the no effect level (NOEL) may be in the range of 100 mg/kg.

Key result

Dose descriptor:

NOEL

Effect level:

100 mg/kg bw/day (nominal)

Based on:

test mat.

Sex:

male

Critical effects observed:

no

Conclusions:

A daily dose of 100 mg/kg was considered to be a no effect level (NOEL).

Executive summary:

The repeated dose toxicity test was done similar to OECD guideline 407. The test item was evaluated in a 2-week (subacute) oral toxicity study. Groups of five male rats were gavaged with 1000 or 100 mg compound/ kg/day for a total of eleven treatments over a 15 day span. A control group of five animals were gavaged with distilled water at a dose of 1000 mg/kg/day. The no effect level (NOEL) may be in the range of 100 mg/kg.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The eye irritation test was performed onto both eyes of 3 rabbits; in one eye of each rabbit, the material was immediately rinsed with water, while in the other eye, the material remained without washing.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,4-diisopropylbenzene

EC Number:

202-826-9

EC Name:

1,4-diisopropylbenzene

Cas Number:

100-18-5

Molecular formula:

C12H18

IUPAC Name:

1,4-bis(propan-2-yl)benzene

Specific details on test material used for the study:

TEST MATERIAL
- name as cited in test report: p-diisopropylbenzene
- Molecular weight: 162 g/mol
- Molecular formula: C12H18
- Physical state: liquid
- Boiling point: 208 °C at 760 mmHg

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability with H+; OH-; Heat, Light, H2: given

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Test system

Vehicle:

unchanged (no vehicle)

Controls:

no

Number of animals or in vitro replicates:

3animals
3 eyes washed, 3 eyes unwashed

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

animal: 1, 2, 3

Time point:

other: 1h

Remarks on result:

other: 3 of 3 animals with washing after treatment and 3 of 3 animals without washing after treatment showed slight irritation, limited to erythema of adnexa, resolved after one hour

Results 2

Irritation parameter:

cornea opacity score

Basis:

animal: 1, 2, 3

Time point:

24/48/72 h

Score:

0

Reversibility:

other: not applicable

Remarks on result:

other: None of the three animals with washing after treatment and none of the three animals without washing after treatment showed Fluorescein stain on the cornea.

Results 3

Irritation parameter:

conjunctivae score

Basis:

animal: 1, 2, 3

Time point:

24/48/72 h

Score:

0

Reversibility:

fully reversible

Remarks on result:

other: None of the three animals with washing after treatment and none of the three animals without washing after treatment showed Fluorescein stain on the adnexa. 1 hour after treatment the adnexa showed a slight erythema.

Results 4

Irritation parameter:

iris score

Basis:

animal:

Time point:

24/48/72 h

Remarks on result:

not measured/tested

Results 5

Irritation parameter:

chemosis score

Basis:

animal:

Time point:

24/48/72 h

Score:

0

Remarks on result:

not measured/tested

Irritant / corrosive response data:

Irritation, limited to erythema of the adnexa, resolved after one hour. No adnexal or corneal staining was observed. Immediate washing was beneficial.

Any other information on results incl. tables

A very minor eye irritant.

 

The compound was a slight eye irritant when tested in six rabbit eyes (three washed and three unwashed). Irritation, limited to erythema of the adnexa, resolved after one hour. No adnexal or corneal staining was observed. Immediate washing was beneficial. 

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Based upon the findings of this study, test item is slightly irritating to the eyes of rabbits. However, the criteria for classification and labelling according to EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No 1272/2008 are not met.

Executive summary:

The eye irritation test was performed onto both eyes of 3 rabbits; in one eye of each rabbit, the material was immediately rinsed with water, while in the other eye, the material remained without washing. Immediately after instillation, slight erythema was observed that was limited to the adnexa, and washing was palliative. No adnexal or corneal staining was observed.

Based on the results observed in the present study, the criteria for classification and labelling according to EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No 1272/2008 are not met.