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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

in vitro cytogenicity / chromosome aberration study in mammalian cells
Type of genotoxicity: chromosome aberration
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Study period:
no data
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data from handbook or collection of data

Data source

Reference Type:
Chromosome aberration tests with Chinese Hamster cells in vitro with and without metabolic activation - a comparative study on mutagens and carcinogens
Ishidate M
Bibliographic source:
Arch. Toxicol. Suppl 4., 41-44, Springer Verlag

Materials and methods

Test guideline
no guideline followed
Principles of method if other than guideline:
CHL cells were treated at different doses for 24 and 48 hours without a metabolic activation system and for 3 hours with S-9 mix from liver homogenate of Wistar rats preterated with PCB. After an additional culture for 24 hours, chromosome preparations were made.
GLP compliance:
not specified
Type of assay:
in vitro mammalian chromosome aberration test

Test material

Constituent 1
Chemical structure
Reference substance name:
Maleic anhydride
EC Number:
EC Name:
Maleic anhydride
Cas Number:
Molecular formula:
Details on test material:
See also SIDS Initial Assessment.


Species / strain
Species / strain / cell type:
Chinese hamster lung fibroblasts (V79)
Metabolic activation:
with and without
Metabolic activation system:
S-9 mix from liver homogenated of Wistar rats treated with PCB
Test concentrations with justification for top dose:
no data

Results and discussion

Test results
Species / strain:
Chinese hamster lung fibroblasts (V79)
Metabolic activation:
not specified
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
D20 = ca. 10E-1 mg/mL
D20: dose (mg/mL) at which chromosome aberrations were detected in 20 % of metaphases observed.
Remarks on result:
other: all strains/cell types tested
Migrated from field 'Test system'.

Applicant's summary and conclusion

Interpretation of results (migrated information): Ambiguous
In Chinsese hamster lung fibroblast cells chromosome aberrations were detected in 20 % of metaphases observed at a concentration range of ca. 10E-1 mg/mL.