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EC number: 700-825-2 | CAS number: -
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The effect of E99434 on the reproduction of Daphnia magna was assessed under semi-static exposure conditions over a period of 21 days using the methods detailed in the OECD Guideline for Testing of Chemicals, No. 211 “Daphnia magna, reproduction test” (2008). As no effects were observed at the water solubility limit and at all loading rates tested, no LOEC can be determined and no NOEC can be derived.
Key value for chemical safety assessment
Additional information
Groups of ten, individually-housed Daphnia were exposed for 21 days to filtered (0.2 μm) water accommodated fractions (WAF) of E99434, prepared from aqueous mixtures with nominal loading rates of 0.954, 3.05, 9.77, 31.3 and 100 mg/L. A control group of twenty Daphnia were exposed to dilution medium alone. The test was initiated with first instar Daphnia that were less than 24-hours old.
Parental mortality:the EC50 value for the parental generation could not be calculated because insufficient mortality occurred, and therefore, must be greater than 100 mg/L. As no effects were observed at the water solubility limit and at all loading rates tested no LOEC can be determined and no NOEC can be derived.
Sub-lethal effects on parental generation:based on analysis of the lengths of surviving adults after 21 days of exposure, no adverse affects on growth were observed. As no effects were observed at the water solubility limit and at all loading rates tested no LOEC can be determined and no NOEC can be derived.
Reproduction:compared to the control group, there was no significant difference in the number of neonates produced by surviving parent Daphnia. As no effects were observed at the water solubility limit and at all loading rates tested no LOEC can be determined and no NOEC can be derived. Under the conditions of the test, the EC10, EC20 and EC50 values for reproduction could not be calculated but must be >100 mg/L.
The time taken to produce the first brood of neonates was not adversely affected at any test concentration; consequently, as no effects were observed at the water solubility limit and at all loading rates tested no LOEC can be determined and no NOEC can be derived.
Under the conditions of the test, the EC10, EC20 and EC50 values for reproduction could not be calculated but must be >100 mg/L.
The time taken to produce the first brood of neonates was not adversely affected at any test concentration; consequently, as no effects were observed at the water solubility limit and at all loading rates tested no LOEC can be determined and no NOEC can be derived.
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