A mixture of: sodium/potassium 7-[[[3-[[4-((2-hydroxy-naphthyl)azo)phenyl]azo]phenyl]sulfonyl]amino]-naphthalene-1,3-disulfonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 August 1992 to 19 November 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Code number: FAT45155/B
Batch number: TV1
Appearance: solid
Solubility: ~100g/l (in water) [estimation]
Storage: room temperature + light protected
Expiration date: 15.5.1997

Analytical monitoring:

yes

Details on sampling:

Test evaluations were done according to the guidelines by visual assessment of mobility of the daphnids after 24 and 48 hours.

Vehicle:

no

Details on test solutions:

The test concentrations were 10, 18, 32, 58, 100, 180 mg/L nominal.

Test organisms (species):

Daphnia magna

Details on test organisms:

The daphnids were bred in the laboratories of the Division Textile Dyestuffs, Product Ecology under standardized conditions. For the test, animals with age of less than 24 hours were used. The quality of the daphnids will be checked at least every 3 months. For each concentration, 20 Daphnia magna were used.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

136 mg/L

Test temperature:

21 °C

pH:

7.9

Dissolved oxygen:

≥96 %

Salinity:

no data

Nominal and measured concentrations:

nominal: 10, 18, 32, 58, 100, 180 mg/L
measured: 101.7-110.0 % of the nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL baker
- Test medium for each test vessel: 80 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2 replicates
- No. of vessels per control (replicates): 2 replicates

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

< 11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

28.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 185 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobility was observed during test duration in the control.

Results with reference substance (positive control):

24 h EC50: 0.8-1.5 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of FAT 45155/B in Daphnia acute toxicity test was 28.1 mg/L upon 48 hours of exposure.

Executive summary:

In a GLP-compliant study, the acute toxicity of FAT 45155/B to Daphnia magna was determined in a 48-hour static test according OECD Guideline 202 and EU method C 2. The nominal concentrations tested were 10, 18, 32, 58, 100, 180 mg test article/l, and a control without any additions. No immobility was observed during test duration in the control. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 101.7 - 110.0 % of the nominal values, under test conditions the test article was sufficiently stable. Based on the study results, the EC50 of FAT 45155/B in Daphnia acute toxicity test was 28.1 mg/L upon 48 hours of exposure.

Description of key information

48 h EC50 of test substance in Daphnia acute toxicity test was 28.1 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

28.1 mg/L

Additional information

In a GLP-compliant study, the acute toxicity of FAT 45155/B to Daphnia magna was determined in a 48-hour static test according OECD Guideline 202 and EU method C 2. The nominal concentrations tested were 10, 18, 32, 58, 100, 180 mg test article/l, and a control without any additions. No immobility was observed during test duration in the control. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 101.7 - 110.0 % of the nominal values, under test conditions the test article was sufficiently stable. Based on the study results, the EC50 of FAT 45155/B in Daphnia acute toxicity test was 28.1 mg/L upon 48 hours of exposure.