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EC number: 219-941-5 | CAS number: 2579-20-6
A study was performed to assess the ready biodegradability of the test material in an aerobic aqueous media. The method followed that described in the OECD Guidelines for Testing of Chemicals (1992) No 301 B, "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC).
The test material was exposed to activated sewage sludge micro-organisms at a concentration of 10 mg C/L with culture medium in sealed culture vessels in the dark at 21°C for 28 days. The degradation of the test material was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the standard material, sodium benzoate, together with a toxicity control were used for validation purposes.
The test material attained 29% degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301 B.
OECD test 301B: Modified Sturm Test - 29% degradation after 28 days therefore substance not considered readily biodegradable.
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