Tetrasodium 5-[4-chloro-6-(N-ethyl-anilino)-1,3,5-triazin-2-ylamino]-4-hydroxy-3-(1,5-disulfonatonaphthalen-2-ylazo)-naphthalene-2,7-disulfonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26.09.1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: ETAD method No. 103

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: >100 g/l
- Appearance: Solid

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

other: Sludge from a Husman laboratory apparatus

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20 °C

Nominal and measured concentrations:

- Nominal test concentration: 1, 3.2, 10, 32 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol (RIEDEL- DE HAEN AG., No.62493)

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

To calculate the inhibitory effect of a test substance, its respiration rate is expressed as a percentage of the mean of two control respiration rates.
IC50 was calculated to be >100 mg/L.No inhibition was seen at any of the test concentrations.

Results with reference substance (positive control):

The IC50 of the reference substance 3,5-Dichlorophenol is: 20 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The IC50 of FAT 40406/A was determined at >100 mg/L.

Executive summary:

The IC₅₀ of FAT 40406/A was determined in a respiration inhibition test performed according to OECD 209, correspondig to ETAD-Method No.103. In this study, a mixed culture of bacteria (sludge of a Husman apparatus) was exposed to 1, 3.2, 10, 32 and 100 mg of test substance/L for 3 h. Positive control was used was 3,5- Dichlorophenol. No inhibition was seen at any of the test concentrations after the incubation period of 3 h. Hence, IC₅₀ was estimated to be >100 mg/L. The IC₅₀ of positive control was estimated to be 20 mg/L.

Description of key information

The 3-h EC50 of Reactive Red 245 was found to be >100 mg/L in microorganisms.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

Additional information

The IC₅₀of Reactive Red 245 was determined in a respiration inhibition test performed according to OECD 209, correspondig to ETAD-Method No.103. In this study, a mixed culture of bacteria (sludge of a Husman apparatus) was exposed to 1, 3.2, 10, 32 and 100 mg of test substance/L for 3 h. Positive control was used was 3,5- Dichlorophenol. No inhibition was seen at any of the test concentrations after the incubation period of 3 h. Hence IC₅₀ was estimated to be >100 mg/L. The IC₅₀ of positive control was estimated to be 20 mg/L.