Tetrasodium 5-[4-chloro-6-(N-ethyl-anilino)-1,3,5-triazin-2-ylamino]-4-hydroxy-3-(1,5-disulfonatonaphthalen-2-ylazo)-naphthalene-2,7-disulfonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25.09.90 to 23.10.90

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

Version / remarks:

Modified AFNOR test

Deviations:

yes

Remarks:

Inoculum: effluent of a domestic sewage treatment plant

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Version / remarks:

Modified AFNOR test

Deviations:

yes

Remarks:

Inoculum: effluent of a domestic sewage treatment plant

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Name: FAT 40406/A
Purity: 89.3 %
Appearance: Solid

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility and stability of the test substance in the solvent/vehicle: >100 g/L

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

- Strain/Inoculation: Mixture of polyvalent bacteria collected on the same day as the day testing started (1990-09-25). Effluent of a domestic sewage treatment plant (ARA-Rhein)
- Bacteria concentration: 7.0E+06/mL

Duration of test (contact time):

28 d

Initial conc.:

40 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

- Experimental Conditions
- Water/Nutrient: According to OECD Method 301A
- Temperature: 23 ± 1 °C
- Lighting: Indirect daylight
- Estimation technique: TOC/DOC SHIMADZU TOC-500

Reference substance:

other: glucose (Merck No. 8342)

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

The level of biodegradation is the percentage elimination of the organic carbon. The test substance was found to be not biodegradable within 28 days under the conditions of the test.

Results with reference substance:

Biodegradation corrected by the blank control and measured as DOC (mg/L) for the reference substance (glucose) is: 100 % (28th day)

Inhibition control: No inhibition on the activity of the bacteria can be found.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

FAT 40406/A is not readily biodegradable (0 % DOC) under the test conditions.

Executive summary:

In a ready biodegradability test performed according to OECD Guideline 301A (modified AFNOR test), biodegradation of the test substance by microorganisms was determined. In this study, a mixture of polyvalent bacteria (7 x 10⁶/ml) from an effluent of a domestic sewage treatment plant (ARA-Rhein) was exposed to 40 mg/L DOC of test substance for 28 days.

After 28 days no biodegradation was observed. The results from inhibitory control showed that no inhibition of the activity of the bacteria can be found.

Description of key information

Reactive Red 245 is not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

In a key study, ready biodegradability test performed according to OECD Guideline 301A (modified AFNOR test), biodegradation of the test substance by microorganisms was determined. In this study, a mixture of polyvalent bacteria from an effluent of a domestic sewage treatment plant (ARA-Rhein) was exposed to 40 mg/L DOC of test substance for 28 days. After 28 days no biodegradation was observed. The results from inhibitory control showed that no inhibition of the activity of the bacteria can be found. In addition, basis of suppourting studies the biochemical oxygen demand (BOD₅) after 5 d was 0 mg O₂/g. Comparing this with the COD of FAT 40406/A is 923 mg O₂/g, the BOD₅/COD ratio is 0. Hence, FAT 40406/A can be considered to be not readily biodegradable.