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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
not required

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
source: Shanghai Longhua Sewage Plants
activity and sensitivity of the microbial innoculum was tested with copper sulfate

Study design

Limit test:
yes
Total exposure duration:
30 min

Test conditions

Test temperature:
20°C
pH:
6-8
Nominal and measured concentrations:
nonimal concentration 100 mg/l
Details on test conditions:
two replicates
Contact between innoculum and the test substance: 30 min
Respiration rate measured over a period of 10 min
Reference substance (positive control):
yes
Remarks:
copper sulfate

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC50 (30 min) = 39.8 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Test substance has no inhibitory effect on activated sludge respiration under these test conditions.