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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Test substance was mixed into the test water as homogeneously as possible by ultrasonic treatment for 15 minutes and stirring for 4 hours at room temperature in the dark to dissolve a maximum concentration of the test substance in the dispersion. The supersaturated stock dispersion was filtered through a 0,45 micrometer membrane filter. The undiluted filtrates were used as test stock solutions.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
10 - 250 mg CaCo3 per Liter
Test temperature:
23
pH:
8
Nominal and measured concentrations:
Nominal concentration: 100 mg/l
measured concentration in mg/l:
test start test end mean value
3.58 1.24 2.41
Details on test conditions:
daily test medium renewal
light-dark cycle: 12 h : 12h
fish were not fed during test
Reference substance (positive control):
yes
Remarks:
potassium dichormate

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Results with reference substance (positive control):
24h-LC50 : 200 - 400 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Test substance has no acute toxic effect on zebra fish up to its solubility limit.