Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report Date:
2006

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Test substance was mixed into the test water as homogeneously as possible by ultrasonic treatment for 15 minutes and stirring for 4 hours at room temperature in the dark to dissolve a maximum concentration of the test substance in the dispersion. The supersaturated stock dispersion was filtered through a 0,45 micrometer membrane filter. The undiluted filtrates were used as test stock solutions.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
10 - 250 mg CaCo3 per Liter
Test temperature:
23
pH:
8
Nominal and measured concentrations:
Nominal concentration: 100 mg/l
measured concentration in mg/l:
test start test end mean value
3.58 1.24 2.41
Details on test conditions:
daily test medium renewal
light-dark cycle: 12 h : 12h
fish were not fed during test
Reference substance (positive control):
yes
Remarks:
potassium dichormate

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
24h-LC50 : 200 - 400 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Test substance has no acute toxic effect on zebra fish up to its solubility limit.