Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-816-9 | CAS number: 74-86-2
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, non-guideline animal experimental study, published in peer reviewed literature, limitations in reporting but otherwise adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Destruction of cytochrome P-450 by vinyl fluoride, fluroxene and acetylene. Evidence for a radical intermediate in olefin oxidation
- Author:
- Ortiz de Montellano PR, Kunze KL, Beilan HS and Wheeler C
- Year:
- 1 982
- Bibliographic source:
- Biochemistry, 21, 1331-1339
Materials and methods
- Objective of study:
- metabolism
- Principles of method if other than guideline:
- The interaction of acetylene with hepatic P-450 was investigated in vitro and in vivo
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Acetylene
- EC Number:
- 200-816-9
- EC Name:
- Acetylene
- Cas Number:
- 74-86-2
- Molecular formula:
- C2H2
- IUPAC Name:
- acetylene
- Details on test material:
- - Source: Matheson Chemical Co., Newark, CA, USA
- No further details
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight: 250±25 g
- No further details
Administration / exposure
- Route of administration:
- inhalation: gas
- Vehicle:
- other: air
- Details on exposure:
- Animals were pre-treated for 4 days with 80 mg/kg/day phenobarbital in aqueous solution by injection.
- Details on dosing and sampling:
- - In-vitro experiment: pre-treated rats were decapitated and hepatic microsomes were prepared
- Acetylene was mixed with air and incubated with the microsomes for up to 30 min, control incubations were performed
- The Cytochrome P-450 activity was measured using a spectrophotometric method
- Abnormal porphyrins were purified and isolated by chromatography and then characterised using MS and NMR
- In-vivo experiment: rats were exposed to acetylene by inhalation, P-450 activity and porphyrins were analysed as above
Results and discussion
Metabolite characterisation studies
- Metabolites identified:
- yes
- Details on metabolites:
- The identity of the abnormal porphyrin was determined to be N-(2-oxoethyl) protoporphyrin IX; this is probably generated by reaction of a free radical metabolite of acetylene formed during oxidation by cytochrome P-450.
Applicant's summary and conclusion
- Executive summary:
Oxidation of acetylene by cytochrome P-450 results in a reactive metabolite which reacts with the prosthetic heme resulting in loss of enzyme activity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
